Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
The role of DIF-1 signaling in Dictyostelium development.
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A mutant blocked in DIF-1 biosynthesis lacks pstO cells, identifying DIF-1 as the pstO inducer and establishing the developmental role of the DIF-1 pathway.
"a mutant blocked in the biosynthesis of DIF-1, a chlorinated signal molecule proposed to induce differentiation of both major prestalk cell types"
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pstO cell differentiation is restored by exogenous DIF-1, showing DIF-1 acts as the diffusible pstO inducer.
"Normal pstO cell differentiation and their patterning in the slug are restored by development on a uniform concentration of DIF-1"
DIF-1 induces the basal disc of the Dictyostelium fruiting body.
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The dmtA-null methyltransferase mutant, blocked in the last step of DIF-1 synthesis, has delayed tip formation and fewer prestalk-O cells.
"The dmtA(-) methyltransferase mutant, blocked in the last step of DIF-1 synthesis, resembles the stlB(-) mutant but has delayed tip formation and fewer prestalk-O cells"
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DIF-1 biosynthesis is completed by O-methylation catalyzed by the DmtA methyltransferase.
"and then methylated to produce DIF-1, with methylation catalyzed by the DmtA methyl transferase"
Early nucleolar disorganization in Dictyostelium cell death.
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DmtA-null cells, which cannot synthesize DIF-1, were used to show that developmental cell death can be induced by exogenous DIF-1 independently of endogenous DIF-1.
"DH1.DmtA- mutant cells (Figure 2) unable to synthesize DIF-1"
The biosynthesis of differentiation-inducing factor, a chlorinated signal molecule regulating Dictyostelium development.
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The DIF-1 methyltransferase is soluble, SAM-dependent, and strongly prefers dichloro-THPH (des-methyl-DIF-1) as substrate.
"The methyltransferase is soluble, uses S-adenosyl-L-methionine as a co-substrate, has a Km for dichloro-THPH of about 1.1 microM, and strongly prefers this substrate to close analogues"
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DIF-1 biosynthesis from the THPH polyketide requires both chlorination and methylation steps.
"Conversion requires both chlorination and methylation of THPH, and enzymatic activities able to do this exist in cell lysates"