Ckmt2 review notes

Evidence summary

• [UniProtKB:P09605] UniProt describes Ckmt2 as reversibly transferring phosphate between ATP and creatine/phosphocreatine for energy transduction.
• PMID:3972849 The fetched GOA file uses this publication for cardiolipin binding, which is kept as non-core membrane-context support rather than the defining function.
• PMID:8086475 The fetched GOA file uses expression evidence for heart and skeletal muscle development terms; these are marked over-annotated because expression/developmental context is not the enzyme core function.

Curation decisions

• Core function: mitochondrial creatine kinase S-type (creatine kinase activity, GO:0004111).
• Specific catalytic activities were accepted; broad parent terms were modified to the specific activity where possible.
• Localization, cofactor/binding, and phenotype-level annotations were retained only as non-core unless directly tied to the enzymatic role.

Re-review 2026-10-04

GOA changes. None in substance: the refreshed GOA has the same 14 rows (3 IBA, 8 IEA, 1 IDA cardiolipin binding from PMID:3972849, 2 IEP developmental rows from PMID:8086475). No PENDING, no retired rows.

Row audit. No action changed (5 ACCEPT, 3 KEEP_AS_NON_CORE, 4 MODIFY of generic catalytic/kinase/transferase parents to GO:0004111 creatine kinase activity, 3 MARK_AS_OVER_ANNOTATED developmental rows). The developmental rows rest on expression timing only PMID:8086475; that is not participation in tissue development, so MARK_AS_OVER_ANNOTATED stands.

Stale quotes fixed. The refreshed UniProt record no longer has DR GO lines, so ten supporting_text quotes of the form "GO; GO:0004111; F:creatine kinase activity; IBA:GO_Central." no longer matched the source. They now quote the record's CATALYTIC ACTIVITY ("Reaction=creatine + ATP = N-phosphocreatine + ADP + H(+);"), SUBCELLULAR LOCATION, TISSUE SPECIFICITY and MISCELLANEOUS ("Mitochondrial creatine kinase binds cardiolipin.") lines. The cardiolipin-binding IDA row also cites its own paper PMID:3972849. The IEA skeletal-muscle-development row now cites the expression data it derives from.

Description. Rewritten as standalone biology; removed "The review accepts ... marks ... as non-core or over-annotated".

Open questions. None new.