Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
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PA2685 (then vgrG4, now vgrG1c) and PA0091 are the most abundant proteins of the on-state H1-T6SS secretome; the double deletion abolishes Hcp1 secretion, unlike deletion of the Tse cargo proteins.
"Western blot analysis revealed that Hcp1 secretion was abolished in both the ΔclpV1 and ΔvgrG1 ΔvgrG4 strains, however it was unaffected by tse deletion"
Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins.
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VgrG1c is secreted in a ClpV1-dependent way, forms multimers, and is redundantly required with VgrG1a for Hcp1 secretion.
"In conclusion, VgrG1a and VgrG1c are essential for efficient Hcp1 secretion but can function independently."