Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Human METTL20 is a mitochondrial lysine methyltransferase that targets the β subunit of electron transfer flavoprotein (ETFβ) and modulates its activity.
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Methylation of ETFβ by METTL20 reduces the ability of ETFβ to receive electrons from GCDH (and MCAD), establishing that GCDH is an ETF-linked dehydrogenase that donates electrons to the electron-transfer flavoprotein.
"methylation reduced the ability of ETFβ to receive electrons from the dehydrogenases MCAD and GCDH"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Lysine catabolism reprograms tumour immunity through histone crotonylation.
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GCDH is the crotonyl-CoA-producing enzyme of the lysine catabolic pathway; its depletion abolishes lysine-induced histone crotonylation.
"GCDH depletion abolished increased Kcr induced by L-lysine supplementation"
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A minor nuclear pool of GCDH (>20% of total) partners with CBP to promote histone lysine crotonylation, while the majority co-localizes with the mitochondrial marker COX IV.
"GCDH most frequently co-localized with the mitochondrial marker COX IV, but >20% of total GCDH localized to nuclei in GSCs"
Cloning of glutaryl-CoA dehydrogenase cDNA, and expression of wild type and mutant enzymes in Escherichia coli.
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Human GCDH cDNA was cloned and expressed with glutaryl-CoA dehydrogenase activity and kinetic constants similar to the purified porcine enzyme; a disease mutant had <1% activity and the alternatively spliced short isoform is inactive.
"Purified expressed human glutaryl-CoA"
glutaryl-CoA + FAD => crotonyl-CoA + FADH2 + CO2
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GCDH catalyses glutaryl-CoA + FAD to crotonyl-CoA + FADH2 + CO2 in the mitochondrial matrix as a homotetramer lacking the 44-residue transit peptide.
"The active enzyme is a tetramer of GCDH polypeptides lacking a 44-residue aminoterminal mitochondrial targeting sequence."
Kinetic mechanism of glutaryl-CoA dehydrogenase.
Functional characterization of rat glutaryl-CoA dehydrogenase and its comparison with straight-chain acyl-CoA dehydrogenase.