Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
A putative long noncoding RNA-encoded micropeptide maintains cellular homeostasis in pancreatic β cells.
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The TUNAR lncRNA encodes a conserved 48-aa single-pass transmembrane micropeptide (BNLN) that localizes to the ER of beta cells.
"BNLN contains a single-pass transmembrane domain"
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BNLN binds SERCA3, with the interaction increased at high glucose.
"One protein enriched in BNLN-FLAG pull-down compared to the GFP-FLAG pull-down was sarco-endoplasmic reticulum Ca2+-ATPases 3 (SERCA3; Table S2)"
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BNLN overexpression lowers ER Ca2+ and enhances glucose-stimulated insulin secretion in rat, mouse and human beta cells/islets.
"Overexpression of BNLN lowered ER calcium levels, maintained ER homeostasis, and"
TUNAR lncRNA Encodes a Microprotein that Regulates Neural Differentiation and Neurite Formation by Modulating Calcium Dynamics.
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Mouse pTUNAR localizes in the ER membrane and co-immunoprecipitates with SERCA2.
"by co-immunoprecipitation experiments we could demonstrate that pTUNAR and SERCA2 physically interact"
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pTUNAR overexpression increases ER Ca2+ store and accelerates cytosolic Ca2+ clearance, suggesting SERCA2 activation.
"our results suggest that pTUNAR is an activator of SERCA2, such as DWORF"
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Start-codon knockout of pTUNAR in mESCs promotes neural differentiation; overexpression reduces neurite formation.
"pTUNAR defficiency in mouse embryonic stem cells promotes their differentiation towards the neural lineage both in vitro and in vivo"
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pTUNAR lacks the regulin xLFxxF motif and has no apparent similarity to regulins or DWORF.
"pTUNAR's transmembrane domain does not contain the xLFxxF motif shared by all regulins"