Annotation inferences using phylogenetic trees
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PAINT node PTN000112207 (PHS1/HACD clade) propagates ER membrane localization, 3-hydroxyacyl-CoA dehydratase activity, fatty acid elongation, VLCFA and sphingolipid biosynthesis to PAS2.
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Pasticcino2 is a protein tyrosine phosphatase-like involved in cell proliferation and differentiation in Arabidopsis.
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PAS2 encodes the Arabidopsis PTP-like (PTPL) protein with a mutated PTP active site; it complements the lethal yeast homolog deletion.
"The absence of yeast PAS2 homolog can be functionally replaced by the Arabidopsis PAS2 protein"
Arabidopsis PASTICCINO2 is an antiphosphatase involved in regulation of cyclin-dependent kinase A.
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PAS2 binds Tyr-phosphorylated CDKA;1 and its loss increases CDKA;1 activity; overexpressed PAS2:GFP appears cytosolic in dividing cells and partly nuclear in differentiated cells.
"Loss of the PAS2 function dephosphorylated Arabidopsis thaliana CDKA;1 and upregulated its kinase activity."
The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development.
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PAS2 is the elongase 3-hydroxyacyl-CoA dehydratase: reciprocal complementation with yeast PHS1, accumulation of 3-hydroxyacyl-CoAs in pas2-1, ER localization and BiFC interaction with CER10, embryo lethality of null alleles.
"the defective elongation cycle resulted in the accumulation of 3-hydroxy-acyl-CoA intermediates, indicating premature termination of fatty acid elongation and confirming the role of PAS2 in this process"
Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.
Arabidopsis Bax inhibitor-1 interacts with enzymes related to very-long-chain fatty acid synthesis.
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AtBI-1 co-purifies with AtELO2, KCR1, PAS2, CER10 and AtCb5-D.
"AtBI-1 forms a complex with AtELO2, KCR1, PAS2, CER10, and AtCb5-D"
The PASTICCINO genes of Arabidopsis thaliana are involved in the control of cell division and differentiation.
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Isolation of pasticcino mutants with cytokinin-enhanced uncoordinated cell division and altered embryo/leaf/root development.
"They display uncoordinated cell divisions which are enhanced by cytokinin."
Falcon deep research report for PAS2 (At5g10480)
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Synthesizes PAS2 as the ER 3-hydroxyacyl-CoA dehydratase of the VLCFA elongase; notes a reported Golgi pool associated with cellulose synthase complexes (Zhu et al. 2020) and cytokinesis defects in pas2-1.
"its primary experimentally validated function is dehydratase activity rather than phosphatase activity"