This guide provides source locators and scope limits for the review. It is an authored explanation, not independent experimental support. Publication identifiers and cache flags remain those of the genuine downloaded sources. A full-text flag is not proof that the cache includes every experimental section; separately recovered originals are distinguished below.
PMID:21163962(https://doi.org/10.1126/science.1195380): human full-length 1–1616 and truncated constructs, alongside separately identified mouse complexes, establish CpG substrate chemistry and autoinhibition. The Results kinetics do not support an invariant twofold substrate preference. PMID:26070743(https://doi.org/10.1016/j.jmb.2015.06.001): Methods describe human 351–1600, tag removal and a C-terminal cloning extension; the LC-MS assay is independent of the crystal structure. PMID:36414620(https://doi.org/10.1038/s41467-022-34779-4): Results and Methods distinguish human DNMT1 351–1616, isopeptide-linked structural H3Ub2 and disulfide kinetic analogs, human rescue and Xenopus replication extracts. Paired K18/K23 recognition is supported; the K14-specific original remains inaccessible.
The human heterochromatin original provides Methods on pp. 37175–37178 and loss/rescue Results on pp. 37179–37183. No isolated catalytic-dependence claim is made. GO:0051718 is a specific child of cytosine-5 methyltransferase activity. The three retained process terms have no pairwise typed ancestor relationship; distinct biological support is still required and is given in the review.
The DNMT1–LANA original is positive for DNMT1 despite its DNMT3A-focused title. UHRF1 refinements use independently purified human DNMT1/UHRF1 experiments; mouse Np95 and human DNMT3B experiments are kept separate. AKT1 refinement uses original Fig. 3 and the main-text description of Supplementary Fig. 5; the supplementary figure pixels were not recovered. The PICK1 source has exact positive MINT two-hybrid/co-IP records; residues 245–446 are a sufficient-region feature, not a natural isoform identity. Native SIRT1/EED/EZH2/CTNNB1/PARP1/DCAF5 complexes are retained without inferring isolated interfaces. No overall screen-quality statistic substitutes for an unavailable target row.
PAINT PTHR10629 places P26358 below PTN000066774, PTN000066775 and PTN000836042. Current IBD assertions cover methyltransferase activity, DNA binding, CpG-mediated repression and nucleus. The target's own experimental evidence is legitimate descendant grounding, not circularity. Mouse P13864 donor records were traced to specific original evidence; missing historical germ-cell-nucleus evidence stays unresolved. The unchanged historical identities and their present-day source counterparts are recorded separately.
The supported HPA DNMT1 record identifies nucleoplasm by immunofluorescence. Image pixels were not independently read. Nucleoplasm excludes chromosomes by definition, but the positive HPA summary is not a chromosome-exclusion experiment. Both nucleoplasm and chromatin are therefore preserved in the core and in five broad nuclear-row refinements. The sixth nuclear row, from PMID:24492612, retains its more precise pericentric heterochromatin pool alongside nucleoplasm; actual Results and Figure 4B caption identify endogenous human DNMT1/PHF20L1/5mC in HeLa pericentric heterochromatin. This descendant already covers chromatin. The source-specific broad-resolution and natural-product limits remain explicit. Current chromatin is part_of the live chromosome term, and neither chromatin nor pericentric heterochromatin has a typed nucleus ancestor; biological nuclear localization is not inferred from a nonexistent edge. Reactome entity 212181 explicitly represents human P26358 1–1616 in nucleoplasm. Its paths through defined set 212315, complex 212288 and catalyst 212303 were checked. SUMOylation and miR-21 regulation do not make DNMT1 the modifying enzyme or its own RNA substrate. The location belongs to the explicitly represented protein entity. MitoCoP/OpenCell extraction records raw hashes, public downloads and reproducible target selection.
The following flags describe the effective protected or genuinely added cache. The final column records what was actually accessed, including separate originals. No unavailable full text is represented as read.
| PMID | Cache full-text flag / method | Actual access and limits |
|---|---|---|
| 10888872 | false / not recorded | Abstract only; the original experimental sections were not recovered. The abstract reports DNMT1 amino-terminal HDAC2/DMAP1 binding and replication-foci targeting. |
| 15161933 | false / not recorded | Abstract and exact MINT-3296749 DNMT1–YWHAZ pull-down record were checked; the full original was not recovered. No target-specific localization or stability consequence is established by this access scope. |
| 16357870 | false / not recorded | Abstract and exact EED/EZH2 interaction records were checked; full original and the listed erratum text were not recovered. Complex association is retained without a purified binary-interface claim. |
| 16791210 | false / not recorded | The cached abstract describes endogenous-tag human nuclear-protein imaging but does not individually identify DNMT1 dynamics. The curated nuclear observation is retained with independent localization support; no unseen DNMT1 time course is asserted. |
| 16983096 | true / html | Separately recovered original Results and Methods show DNMT1-positive GST-LANA/in-vitro-translation and cotransfection assays. DNMT3A is stronger and has distinct retargeting behavior. |
| 17673620 | false / not recorded | Abstract-only cache and independent human DNMT1/UHRF1 biochemistry in PMID:21745816 support the association. This paper's full experimental sections were not recovered. |
| 17934516 | false / not recorded | The abstract explicitly reports placental-library two-hybrid, purified-protein pull-down and human co-IP; full experimental sections were unavailable. Independent human binary evidence is also present in PMID:21745816. |
| 17972916 | true / html | Selected original human HeLa co-IP and recombinant RIP140/in-vitro-translated DNMT1 binding experiments were read. Mouse adipocyte Ucp1 promoter regulation is a separate context. |
| 18413740 | true / html | Selected original Results and Methods were read. Human BAG1 expression and BORIS-associated chromatin effects are positive; the study does not establish a DNMT1 sequence-specific transcription-factor activity. |
| 18754681 | false / not recorded | Cached abstract and authentic publisher-indexed original Results/Methods were read. Purified human DNMT1/CXXC constructs support unmethylated-CpG recognition and marked activity reduction after selected mutations or deletion; residual activity was measured. |
| 19282482 | true / html | Selected original Results and Methods establish human DNMT1–SET7 association, recombinant binding and K142 methylation; DNMT1 is the substrate of SET7. |
| 19450230 | false / not recorded | The abstract directly reports mapped SUMOylation and enhanced DNMT1 activity. Full target-pair experimental sections were not recovered, so the UBE2I binding assertion remains unresolved. |
| 19798101 | true / xml | Selected full-cache Methods and Results were read. Mouse Np95/Dnmt1 experiments are distinguished from a separate human DNMT3B construct; host cell identity does not determine construct species. |
| 20820192 | true / html | Selected separately recovered original Results and Methods were read. BRCA1 loss lowers DNMT1 expression in the tested mouse/human systems; the earlier opposite-direction summary is corrected. |
| 21151116 | true / html | Selected original Results and Methods were read, including Fig. 3 and the textual description of Supplementary Fig. 5. The supplementary figure pixels were not recovered. Human AKT1/DNMT1 fragment binding and the SET7-bound short substrate peptide have distinct scopes. |
| 21653829 | true / html | Original screen Methods and exact MINT pair records were checked. The DNMT1–PICK1 records specify positive two-hybrid/co-IP and a sufficient DNMT1 region, 245–446; the supplementary table was not recovered. |
| 21745816 | true / xml | Selected complete cached Methods/Results describe purified human DNMT1, USP7 and UHRF1, mapped binary interfaces, nuclear complexes, promoter occupancy and methylation. USP7-dependent stimulation is not DNMT1's unmethylated-versus-hemimethylated substrate preference. |
| 21947282 | true / html | Selected separately recovered original Methods/Results distinguish human reciprocal co-IP, domain mapping and deacetylation of immunopurified DNMT1. These are not an isolated two-purified-protein binding assay. |
| 22094255 | true / html | Selected separately recovered original Results/Methods were read. Human oxidative-damage-dependent complexes contain DNMT1, SIRT1 and Polycomb proteins; individual contacts are not resolved by native capture. |
| 24492612 | true / xml | Selected cached original Results/Methods were read, including methylated-DNMT1 peptide recognition and cellular localization. PHF20L1 is the methyl-reader; DNMT1 is its modified ligand. |
| 24623306 | true / xml | Selected complete cached original Results/Methods establish human DNMT1 depletion, promoter ChIP and INK4-ARF methylation/expression changes. Occupancy is not purified sequence-specific transcription-factor binding. |
| 25544563 | true / html | Original screen Methods/Results were recovered, but the Q77UV9–DNMT1 supplementary pair table was not. HTTP 200 HTML challenges and a non-ZIP archive response were retained as failed access, not original data. |
| 25753001 | true / html | The protected cache contains abstract/introduction/discussion, not complete Results/Methods. Literal human DNMT1–CTNNB1 co-IP/colocalization records were independently checked. |
| 27021683 | false / not recorded | Abstract-only cache; full original was not recovered. The abstract explicitly reports human pulmonary artery smooth-muscle-cell DNMT1-dependent effects. Curated contextual processes are retained without invented construct or assay details. |
| 27728808 | true / html | The protected cache lacks complete experimental sections. Exact human DNMT1–PARP1 co-IP records and the accessible drug/damage discussion were checked. |
| 29691401 | true / xml | Selected original Results/Methods distinguish L3MBTL3 recognition of methylated DNMT1 from DCAF5 recruitment that depends on L3MBTL3. Native AP-MS/co-IP does not alone establish direct DCAF5 recognition. |
| 32051553 | true / xml | Selected original U251 ZNF263 AP-MS/co-IP Results and construct Methods were read. The association does not independently establish direct binary recognition. |
| 34800366 | true / xml | Original importomics Methods and genuine supplementary workbook target rows were read. MitoCoP group 4315 includes canonical and alternative accession groups; group 4316 is separate. No natural-isoform assignment or mitochondrial DNA methylation activity follows. |
| 35271311 | true / html | The genuine author original Methods summary and exact downloadable CSV record were read. The positive YWHAZ-bait DNMT1-prey protein group does not identify a natural isoform or purified direct interaction. |
| 8940105 | false / not recorded | The complete cached abstract was read; full original was not recovered. Historical translation-start constructs are not automatically the three current UniProt splice products. |
| 12145218 | true / html | Selected separately recovered original Results and Methods were read. Human DNMT1 nuclear co-IP and an engineered amino-terminal fragment in COS7 are distinct from current natural products. |
| 26070743 | true / xml | Independent original Methods/Results were read: human residues 351–1600, tag cleavage and a C-terminal cloning extension, crystallography and LC-MS methylation assays. The experimental fragment is not natural isoform 3. |
| 29053958 | false / not recorded | The protected cache is abstract-only. Publisher PDF access failed; the accessible Elsevier response was bibliographic metadata, not full text. The K18/K23 mechanism has independent human support in PMID:36414620; the specific K14 assays remain unresolved. |
| 21163962 | true / xml | Independent original Results/Methods distinguish human and mouse complexes and condition-specific kinetics. Human full-length 1–1616 and truncated constructs were tested; no invariant twofold substrate preference is supported. |
| 36414620 | true / xml | Independent original Methods/Results were read. Human DNMT1 351–1616, isopeptide-linked structural H3Ub2 and disulfide-linked kinetic analogs are distinguished; human cell rescue and Xenopus extract experiments are separate. H3K18/K23 recognition does not establish H3K14 recognition. |
| 20548288 | true / xml | Selected original mouse diet Methods/Results were read. Dnmt1 is included as a measured protein, but the visible DNMT changes emphasize Dnmt3a/3b. Diet-responsive measurements do not establish a Dnmt1-executed amino-acid response. |
| 20573698 | false / not recorded | Authentic publisher-indexed full original Methods/Results were read despite an abstract-only normal cache and direct PDF access failure. Native RIP supports Kcnq1ot1 association; the authors do not exclude protein-mediated recruitment. |
| 21980460 | true / xml | Selected original Methods/Results were read. Gestational BPA and embryonic mouse-brain expression measurements include Dnmt1; they do not perturb Dnmt1 to establish its own response function. |
| 25686699 | false / not recorded | Separately recovered full PMC original Results/Methods were read. Mouse C2C12/repair-context RIP and nuclear-extract RNA pull-down are positive, but not purified direct RNA binding. |
| 10615135 | false / not recorded | Complete normal-cache abstract and mouse IDA source record were checked; full original was unavailable. The abstract reports Dnmt1-associated HDAC activity and repression, not an isolated chromatin-assembly reaction. |
| 15220328 | false / not recorded | Genuine institutional full original Methods/Results were read. Human HCT116 DNMT1-deficient cells and mouse Dnmt1 rescue are distinct. Downstream chromatin changes do not isolate catalytic dependence; cancer-context nuclear-morphology limits are explicit. |
Nine annotation review objects received bounded location or rationale corrections, with all 104 imported source objects and actions unchanged. Replication-fork evidence remains in its existing source-specific row; no fork assay is invented for broad nuclear observations. The complementary H3K18ub and H3K23ub reader cores and the catalytic core remain unchanged. Public provenance uses a normalized portable command while preserving genuine execution times and source hashes. No new reference, publication quote, source-cache edit or history rewrite is introduced.