Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Isolation, partial amino acid sequence, and cellular distribution of heat-shock protein hsp98 from Neurospora crassa.
-
Hsp98 is a prominent N. crassa heat-shock protein with sequence homology to Hsp100/ClpB family members, including S. cerevisiae Hsp104.
"This 28 amino acid sequence from hsp98 has 75% homology with a region of the ClpB protein of Escherichia coli and 86% homology to a 96-kDa protein of Trypanosoma brucei. It also has 71% homology to hsp104 of Saccharomyces cerevisiae."
-
During heat shock, hsp98 was enriched in a microsomal fraction whose high-molecular-weight heat-shock proteins were concentrated in polyribosomes.
"Hsp98 was enriched in the microsomal fraction of heat-shocked cells. Sucrose gradient analysis of this cellular fraction showed that the three major high molecular weight heat-shock proteins (hsp98, 83 and 67) were more concentrated in polyribosomes than in monoribosomes."
UniProtKB reviewed entry for hsp98