Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of genome-wide subcellular localisation of proteins using fluorescent protein tagging in Trypanosoma brucei
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Association of two novel proteins, TbMP52 and TbMP48, with the Trypanosoma brucei RNA editing complex.
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TbMP52 (REL1) identified as a component of the ~1,600 kDa editing complex purified from T. brucei mitochondria.
"These results suggest that TbMP52 and TbMP48 are components of the RNA editing complex."
An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei.
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TbMP52 has RNA ligase activity, is adenylatable, and is essential for editing and bloodstream-form viability.
"Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex."
High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei: RNA editing ligase 1.
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1.2 A structure of the REL1 catalytic domain bound to ATP and Mg2+.
"We report here the crystal structure of the catalytic domain of TbREL1 at 1.2 A resolution, in complex with ATP and magnesium."
Separate insertion and deletion subcomplexes of the Trypanosoma brucei RNA editing complex.
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Tagged REL1 purifies a deletion-editing subcomplex distinct from the REL2 insertion subcomplex.
"A purified TbREL1 subcomplex catalyzed precleaved deletion editing in vitro, while a purified TbREL2 subcomplex catalyzed precleaved insertion editing in vitro."
The Trypanosoma brucei MitoCarta and its regulation and splicing pattern during development.
A Protein Complex Map of Trypanosoma brucei.