Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Ceramide phosphoethanolamine biosynthesis in Drosophila is mediated by a unique ethanolamine phosphotransferase in the Golgi lumen.
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CG33116/dCCS1 localizes exclusively to the ER (S2 and HeLa cells), unlike its paralog CG7149/dCCS3, which is partly at the Golgi.
"dCCS1 and dCCS2 localized exclusively to the ER, whereas dCCS3 and dCCS4 partially co-localized with the Golgi marker GM130"
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CG33116 and CG7149 are fly-lineage duplicates with a single human orthologue.
"CG33116 and CG7149 (46% identity) have only one human orthologue and must therefore have originated from a gene duplication specific to the fly lineage"
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Depleting CG33116 did not reduce CDP-ethanolamine-dependent ceramide phosphoethanolamine synthesis; CG4585/dCCS4 is the CPE synthase.
"Contrary to removal of dCCS1, dCCS2, or dCCS3, depletion of dCCS4 caused a major (∼60%) reduction in CPES activity"
DROME/CG33116/CG33116-uniprot.txt
CG33116 re-review subfamily placement and CAPT motif analysis
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SF71 is an invertebrate EPT1-type subfamily; its vertebrate counterpart SF47 contains SELENOI. CG33116 groups with CG7149 inside the EPT1 lineage, apart from CEPT1/CHPT1/bbc.
"CG33116 is an invertebrate member of the EPT1/SELENOI lineage"