Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Outer membrane c-type cytochromes required for Fe(III) and Mn(IV) oxide reduction in Geobacter sulfurreducens.
-
OmcS was recovered among the most abundant proteins sheared from the outer surfaces of intact cells.
"Two of the most abundant proteins that were easily sheared from the outer surfaces of intact cells were c-type cytochromes."
-
The outer membrane assignment in this paper is a prediction, not a measurement.
"One, designated OmcS, has a molecular mass of ca. 50 kDa and is predicted to be an outer membrane hexaheme c-type cytochrome."
-
Deleting omcS abolishes Fe(III) oxide reduction but leaves soluble acceptors, including Fe(III) citrate, unaffected; expression in trans restores it.
"When either omcS or omcE was deleted, G. sulfurreducens could no longer reduce Fe(III) oxide but could still reduce soluble electron acceptors, including Fe(III) citrate."
-
OmcT cannot substitute for OmcS in Fe(III) oxide reduction.
"genetic studies indicated that G. sulfurreducens could not reduce Fe(III) oxide when omcT was expressed but OmcS was absent"
-
omcS transcripts appear on Fe(III) oxide but not on soluble Fe(III) citrate.
"Transcripts for omcS could be detected during growth on Fe(III) oxide, but not on soluble Fe(III) citrate."
Alignment of the c-type cytochrome OmcS along pili of Geobacter sulfurreducens.
-
Immunogold localised OmcS along the pili at the cell surface.
"Immunogold localization revealed that OmcS, a cytochrome that is required for Fe(III) oxide reduction by Geobacter sulfurreducens, was localized along the pili."
-
The authors inferred from the spacing that OmcS passes electrons from pili to Fe(III) oxide rather than conducting along the pili.
"The apparent spacing between OmcS molecules suggests that OmcS facilitates electron transfer from pili to Fe(III) oxides rather than promoting electron conduction along the length of the pili."
Biochemical characterization of purified OmcS, a c-type cytochrome required for insoluble Fe(III) reduction in Geobacter sulfurreducens.
-
OmcS is loosely bound to the outer surface rather than membrane-integrated.
"OmcS, an abundant c-type cytochrome that is only loosely bound to the outer surface"
-
Purified OmcS is 47015 Da with six low-spin bis-histidinyl hexacoordinated hemes and a midpoint potential of -212 mV.
"Purified OmcS had a molecular mass of 47015 Da, and six low-spin bis-histidinyl hexacoordinated heme groups."
-
Reduced OmcS transfers electrons to insoluble Fe(III) oxide, Mn(IV) oxide and humic substances.
"Dithionite-reduced OmcS was able to transfer electrons to a variety of substrates of environmental importance including insoluble Fe(III) oxide, Mn(IV) oxide and humic substances."
Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers.
-
The conductive extracellular filaments of G. sulfurreducens are polymers of OmcS, not type IV pili.
"G. sulfurreducens nanowires are assembled by micrometer-long polymerization of the hexaheme cytochrome OmcS"
-
Subunits are joined through stacked hemes and through axial coordination of heme by a histidine from the neighbouring subunit.
"The inter-subunit interfaces show unique structural elements such as inter-subunit parallel-stacked hemes and axial coordination of heme by histidines from neighboring subunits."
-
Filaments from wild type are 100-fold more conductive than those from a ΔomcS strain.
"Wild-type OmcS filaments show 100-fold greater conductivity than other filaments from a ΔomcS strain"
-
The wild-type conductive appendages are primarily OmcS filaments.
"the conductive extracellular appendages of wild-type G. sulfurreducens strain are primarily OmcS filaments"
Transcriptomic and genetic analysis of direct interspecies electron transfer.
-
OmcS is essential for direct interspecies electron transfer and its transcript rises 240-fold in DIET cocultures relative to hydrogen-based cocultures.
"Transcript abundance for the pilus-associated cytochrome OmcS, which is essential for DIET but not for HIT, was 240-fold higher in the cocultures with G. metallireducens than in cocultures with P. carbinolicus."
Structure of Geobacter pili reveals secretory rather than nanowire behaviour.
-
OmcS reaches the extracellular fraction of wild-type cells but remains periplasmic without PilA-N.
"Our subcellular localization experiments revealed that both OmcS and OmcZ are present in the extracellular fraction of wild-type cells but remain in the periplasm for ΔpilA-N cells"
Dissecting the Structural and Conductive Functions of Nanowires in Geobacter sulfurreducens Electroactive Biofilms.
The varied roles of pilA-N, omcE, omcS, omcT, and omcZ in extracellular electron transfer by Geobacter sulfurreducens.
-
Deleting omcS impairs ferrihydrite reduction and coculture formation, but the mutant regains the ability to reduce anodes.
"The mutant without omcS showed diminished ability to reduce ferrihydrite and to form the co-culture, but was able to regain its ability to reduce anodes."
PAINT IBD nodes for PTHR30333
Family review of PANTHER PTHR30333