These locators expose the target-level evidence behind the review. They describe selected original text and tables; they do not imply full figure, supplementary-image or raw-data inspection.
| Source | Target evidence and original locator | Limit |
|---|---|---|
| PMID:8613547(https://doi.org/10.1172/JCI118517) | Original Methods “Transient transfection in COS-7 cells” and “Enzyme assay”; Table III compares wild-type and variant human constructs with mock controls. | Coupled activity assay; historical clones were not aligned to the current sequence. |
| PMID:26199317(https://doi.org/10.1093/hmg/ddv280) | Selected Methods and Results distinguish engineered human 38–700 protein, observed noncatalytic sugar contacts and modeled active-site binding. | A fragment is not a natural product; modeling is not observed active-site substrate occupancy. |
| PMID:24837458(https://doi.org/10.1042/BSR20140053) | Results/Fig. 4A text reports tagged GBE1–STBD1 co-immunoprecipitation. | The later recruitment/deprivation experiments concern other enzymes. |
| PMID:36217029(https://pubmed.ncbi.nlm.nih.gov/36217029/) | Supplementary Table 1, sheet B, worksheet row 102: GBE1/Q04446/ENSG00000114480–NSP16, HuSCI-HIS3=1, HuSCI-GFP=0. Methods specify triplicate HIS3 retesting, at least two positive repeats and sequence confirmation. | The GFP assay used a different fusion/plasmid configuration. No target pair in inspected orthogonal N2H Table 3; no infected-cell function. |
The viral partner P0DTD1, PRO_0000449633 is the NSP16 mature chain at residues 6799–7096. Its RNA cap methyltransferase class supports enzyme binding, without transferring catalysis to GBE1. STBD1/O95210 is a glycogen cargo receptor; that identity does not make it a signaling receptor.
The NHLBI original protein table identifies GBE1/NP_000149 with one peptide (table row 449). Its legend assigns source 2 to PMID:19056867(https://pubmed.ncbi.nlm.nih.gov/19056867/). The historical unversioned RefSeq accession is not a sequence-resolved modern isoform claim.
The original PMID:23533145 supplement SI Table 2, row 842, identifies Q04446/GBE1: one protein and unique-peptide counts PCa1/PCa2/NC1/NC2 = 0/0/2/2. Methods describe two analyses per clinical pool. These are not independent donor replicates; contamination and preparation purity remain limits, and zero counts do not establish disease-specific absence.
3322005, 3322016 and 3322057 each use catalyst 71577 → physical entity 71576 → reference 55670, human Q04446 residues 1–702 in cytosol. 3322077 explicitly contains these normal reactions.
3878762 is a FailedReaction: catalyst 9631892 → set 3878772 → F257L/R515C GBE1 members. It links normalReaction 3322016. 3878781 is the disease pathway containing that event. Failed mutant catalysis is kept separate from normal target activity.
The official IPR006048 entry maps to cation binding. Its calcium-site discussion concerns alpha-amylase loop domains, not a GBE1 experiment. The human UniProt record lists carbohydrate ligand features and structures 4BZY/5CLT/5CLW, but no metal-binding keyword or cofactor statement. Selected structural text was read; coordinates were not independently audited. Absence of a recorded metal is not a binding-negative result.
UniProt FUNCTION/CATALYTIC ACTIVITY describe glucan-segment transfer, DOMAIN describes additional carbohydrate recognition, and SUBUNIT assigns a monomer from structural inference. PMID:8463281(https://pubmed.ncbi.nlm.nih.gov/8463281/) independently reports human-cDNA complementation of yeast glc3; only its authentic abstract was inspected.
Original PANTHER19.0 files: tree, alignment, sequence FASTA, node attributes, plus the committed IBD assertions. Human Q04446 is AN42/PTN002536026. Its ungapped sequence matches the 702-residue canonical protein; the tree has 499 nodes and 268 leaves.
| Incoming term | Actual IBD origin | Target relation |
|---|---|---|
| GO:0003844 branching activity | AN20/PTN000040010 | Ancestor of AN42 |
| GO:0005737 cytoplasm | AN20/PTN000040010 | Ancestor of AN42 |
| GO:0005978 glycogen biosynthesis | AN24/PTN000040012 | Ancestor of AN42 |
The target path is AN0 → AN20 → AN21 → AN23 → AN24 → AN25 → AN26 → AN27 → AN28 → AN29 → AN30 → AN31 → AN32 → AN33 → AN34 → AN35 → AN36 → AN37 → AN38 → AN39 → AN40 → AN41 → AN42. No retained target-path negative placement was found. Human self-donor evidence is legitimate descendant grounding. Literal incoming row dates are preserved separately from the later retained IBD dates. This verifies inheritance, not every donor's experimental biology.