Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Glucosinolate engineering identifies a gamma-glutamyl peptidase.
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Identifies GGP1 by reconstituting benzylglucosinolate biosynthesis in N. benthamiana; GGP1 metabolizes the accumulating GSH conjugate and raises glucosinolate yield.
"gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate"
Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis.
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ggp1 ggp3 knockdowns accumulate up to ten glucosinolate-related GSH conjugates and GS-IAN, are reduced in camalexin, and recombinant GGP1/GGP3 hydrolyze these conjugates; both proteins are cytosolic.
"Our results demonstrate that GSH is the sulfur donor in the biosynthesis of glucosinolates and establish an in vivo function for the only known cytosolic plant γ-glutamyl peptidases"
Assigning gene function in biosynthetic pathways: camalexin and beyond.
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Commentary arguing from mutant intermediates, end-product levels, enzyme activity and localization that GGP1 (not the apoplastic GGT1/GGT2) is the camalexin-pathway gamma-glutamyl peptidase.
"we conclude that GGP1 and not GGT1 or GGT2 are part of the camalexin pathway"
A proteomic strategy for global analysis of plant protein complexes.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Protein and metabolite composition of Arabidopsis stress granules.
Glutathione degradation activity of γ-glutamyl peptidase 1 manifests its dual roles in primary and secondary sulfur metabolism in Arabidopsis.
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GGP1 (and GGP3) degrade reduced GSH in vitro with physiological Km; ggp1 mutants accumulate more GSH and have lower glucosinolates, indicating a dual role in primary and secondary sulfur metabolism.
"we suggest that GGP1 and possibly GGP3 play vital roles in both primary and secondary sulfur metabolism"
Crystal structure of plant γ-glutamyl peptidase 1: implications for sulfur metabolism and secondary metabolite biosynthesis.
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Crystal structures of GGP1 reveal a Cys100/His192 catalytic dyad forming a covalent gamma-glutamyl intermediate and an open S1' pocket explaining dual activity on GSH and GSH conjugates.
"An open pocket is present beyond the thiol side chain of cysteine in the S1' subsite, which contributed to the dual activity of GGP1 toward GSH and the GSH conjugates."