Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic Gene Ontology annotation based on Rhea mapping
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProt record Q88DZ5 (RecBCD enzyme subunit RecB)
Deep research report (Falcon/Edison) on P. putida KT2440 recB (Q88DZ5)
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Conserved RecBCD biology supports RecB as the ATP-dependent helicase-nuclease subunit that processes dsDNA ends into recombinogenic 3' ssDNA and loads RecA; pseudomonad genetics (P. syringae recB mutants UV/MMC-sensitive, accumulate fragmented DNA) corroborate the DSB-repair role. No KT2440-specific recB mutant phenotype was recovered, so organism-specific claims are inferred.
"Direct experimental evidence for PP_4673/Q88DZ5 in *P. putida* KT2440 is limited in the retrieved corpus."
Activation of Chi recombinational hotspots by RecBCD-like enzymes from enteric bacteria.
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Cloned P. putida and P. aeruginosa recBCD-like genes restore recombination proficiency and ATP-dependent nuclease activity to an E. coli recBCD deletion but confer neither Chi hotspot activity nor Chi-dependent cleavage.
"Three clones from Pseudomonas aeruginosa and Ps. putida conferred recombination proficiency and ATP-dependent nuclease activity, but neither Chi hotspot activity nor Chi-dependent DNA cleavage."
RecBCD enzyme: mechanistic insights from mutants of a complex helicase-nuclease
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RecBCD is a fast, processive ATP-dependent helicase-nuclease; RecB contributes the 3'-5' motor and a tethered Mg2+-dependent nuclease domain, and Chi recognition switches the enzyme to nick near Chi and load RecA.
All three subunits of RecBCD enzyme are essential for DNA repair and low-temperature growth in the Antarctic Pseudomonas syringae Lz4W
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In a pseudomonad, recB/recC/recD null mutants are UV- and mitomycin C-sensitive and accumulate linear/fragmented chromosomal DNA; RecB ATPase/helicase activity is essential in vivo while the nuclease center is partly dispensable.
"while the nuclease activity of RecB is not so critical in vivo, the ATP-dependent functions of both RecB and RecD are essential"
OpenScientist PSEPK RecBCD end-resection synthesis
Bacterial RecBCD double-strand-end resection module