Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
Fruiting body formation by Bacillus subtilis.
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Demonstrated that B. subtilis forms structured biofilms (fruiting bodies) with preferential sporulation sites
"aerial structures, or fruiting bodies, that served as preferential sites for sporulation"
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Showed fruiting body formation depends on early sporulation regulatory genes including Spo0A
"Fruiting body formation depended on regulatory genes required early in sporulation and on genes evidently needed for exopolysaccharide and surfactin production"
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Found multicellularity preserved in natural isolates but lost in laboratory strains
"The formation of aerial structures was robust in natural isolates but not in laboratory strains, an indication that multicellularity has been lost during domestication of B. subtilis"
The FtsEX ABC transporter directs cellular differentiation in Bacillus subtilis.
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Showed FtsEX transporter is required for proper asymmetric septum formation during sporulation
"in the absence of this transporter, entry into sporulation is delayed and an atypical symmetric septum is formed instead of a polar one"
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Demonstrated that constitutive Spo0A activation suppresses ftsEX sporulation defects
"this phenotype can be suppressed by artificially activating the master regulator of sporulation, Spo0A, or by activating the histidine kinases that function upstream of Spo0A"
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Established FtsEX as upstream of Spo0A in the sporulation hierarchy
"the FtsEX transporter is one of the top components in the hierarchy of factors required to initiate sporulation, and thus it is essential for establishing proper temporal activation of the process"
DegU and Spo0A jointly control transcription of two loci required for complex colony development by Bacillus subtilis.
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Showed DegU and Spo0A jointly regulate yvcA and yuaB transcription for biofilm development
"Activation of transcription from both the yvcA and yuaB promoters requires DegU approximately phosphate, but transcription is inhibited by direct AbrB binding to the promoter regions"
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Demonstrated Spo0A~P relieves AbrB repression by inhibiting abrB expression
"Inhibition of transcription by AbrB is relieved when Spo0A approximately phosphate is generated due to its known role in inhibiting abrB expression"
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Established genetic network linking DegU and Spo0A in biofilm/colony development
"a genetic network dependent on activation of both DegU and Spo0A controls complex colony development by B. subtilis"
Deep research synthesis on Spo0A function
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Spo0A is the master transcriptional regulator at terminus of sporulation phosphorelay
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Phosphorylation of receiver domain enables DNA binding and dimerization
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Graded Spo0A~P levels control cell fate (biofilm vs sporulation)
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Spo0A~P binds 0A-box consensus 5'-TGNCGAA-3' with sequence selectivity
UniProt entry P06534 for Spo0A
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Response regulatory domain (5-123) with phosphorylation site at Asp-56
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HTH DNA-binding motif (199-218)
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Activator of spoIIa operon, repressor of abrB
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Binds 0A-box sequence 5'-TGNCGAA-3'
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Ca(2+) binding based on sequence similarity
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Essential for asymmetric septum formation during sporulation initiation