Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Isochorismate synthase (PchA), the first and rate-limiting enzyme in salicylate biosynthesis of Pseudomonas aeruginosa.
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Purified PchA is a Mg2+-dependent monomeric isochorismate synthase with Km 4.5 uM for chorismate.
"The native enzyme was a 52-kDa monomer in solution, and its activity strictly depended on Mg(2+)."
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PchA concentration limits the rate of salicylate formation.
"The intracellular PchA concentration critically determined the rate of salicylate formation both in vitro and in vivo."
Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa.
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pchBA are necessary for salicylate formation; PchA converts chorismate to isochorismate.
"Salicylate formation was demonstrated in an Escherichia coli entC mutant lacking isochorismate synthase when this strain expressed both the pchBA genes, but not when it expressed pchB alone."