Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The cyclic nucleotide specificity of three cAMP receptors in Dictyostelium.
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cAR3 binds cAMP with two affinity states (~20 and 500 nM in phosphate buffer), distinct from cAR1 and cAR2.
"there were two affinity states of about 30 and 300 nM for cAR1 and 20 and 500 nM for cAR3 but no detectable affinity for cAR2"
Chemoattractant signaling in dictyostelium discoideum.
The COP9 signalosome regulates cell proliferation of Dictyostelium discoideum.
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A COP9 signalosome subunit (CSN5) was identified as a potential interacting partner of a Dictyostelium cell-surface receptor by yeast two-hybrid.
"we have identified the CSN5 subunit as a potential interacting partner of a cell surface receptor of Dictyostelium"
Chemoattractant-mediated Rap1 activation requires GPCR/G proteins.
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Chemoattractant-induced Rap1 activation requires the cAMP receptors cAR1/cAR3 and the G-protein subunit Galpha2.
"The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2"
The cyclic nucleotide specificity of eight cAMP-binding proteins in Dictyostelium discoideum is correlated into three groups.
The Ras association domain of DydA as a specific reporter of activated RasG in Dictyostelium.
Two cAMP receptors activate common signaling pathways in Dictyostelium.
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cAR1 and cAR3 are partially redundant and couple to the same heterotrimeric G protein to mediate cAMP relay and other responses.
"We conclude that the functions of the cAR1 and cAR3 receptors are partially redundant and that both interact with the same heterotrimeric G-protein to mediate these and other responses"
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car1-/car3- double-mutant cells are totally insensitive to cAMP.
"these lines are totally insensitive to cAMP"
Switching of chemoattractant receptors programs development and morphogenesis in Dictyostelium: receptor subtypes activate common responses at different agonist concentrations.
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The Dictyostelium cAMP chemoattractant receptor family comprises four sequentially expressed receptors (cAR1-cAR4).
"The cAMP chemoattractant receptor family of Dictyostelium discoideum is composed of four receptors (cAR1-cAR4), which are expressed sequentially throughout the developmental transition from a unicellular to a multicellular organism"
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cAR3 mediates chemotaxis, adenylyl cyclase activation and actin polymerization and can rescue the car1-/car3- developmental defect.
"assessed the capacity of each to mediate chemotaxis, activation of adenylyl cyclase and actin polymerization, and rescue the developmental defect of car1-/car3- cells"