Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.
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ConE (formerly yddE) is required for conjugation
"conE (formerly yddE) is required for conjugation"
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Conjugative transfer requires conserved ATPase motif of ConE
"conjugative transfer of ICEBs1 requires a conserved ATPase motif of ConE"
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ConE belongs to HerA/FtsK superfamily of ATPases including VirB4
"ConE belongs to the HerA/FtsK superfamily of ATPases, which includes the well-characterized proteins FtsK, SpoIIIE, VirB4, and VirD4"
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ConE-GFP localizes to membrane predominantly at cell poles
"a ConE-GFP (green fluorescent protein) fusion associated with the membrane predominantly at the cell poles in ICEBs1 donor cells"
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Membrane and polar localization requires other ICEBs1 gene products
"At least one ICEBs1 product likely interacts with ConE to target it to the membrane and cell poles"
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ICEBs1 is positioned near midcell when integrated and near poles after excision
"When integrated in the chromosome, ICEBs1 was located near midcell along the length of the cell... Following excision, ICEBs1 was more frequently found near a cell pole"