"Direction is VIRUS-SPECIFIC, and the preponderance of decisive perturbation evidence points to SIRT2 acting as a host DEPENDENCY (pro-viral) factor"

"Both decisive infectious-progeny studies point pro-viral."

"the primary literature does not support a uniform host-restriction role"

"The antiviral phenotypes are **downstream, context-dependent consequences** of this deacetylase activity, not a distinct "defense response to virus" function."

"Here five human sirtuin cDNAs are characterized. The SIRT1 sequence has the closest homology to the S. cerevisiae Sir2p."

"Recombinant E. coli cobB and human SIRT2 sirtuin proteins were able to cause radioactivity to be transferred from [32P]NAD to bovine serum albumin (BSA)."

"The 1.7 A crystal structure of the 323 amino acid catalytic core of human SIRT2, a homolog of yeast Sir2, reveals an NAD-binding domain, which is a variant of the Rossmann fold, and a smaller domain composed of a helical module and a zinc-binding module."

"The 1.7 A crystal structure of the 323 amino acid catalytic core of human SIRT2, a homolog of yeast Sir2, reveals an NAD-binding domain, which is a variant of the Rossmann fold"

"a smaller domain composed of a helical module and a zinc-binding module"

"SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo."

"SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase."

"Our functional studies of human SIRT2, a homolog of the product of the yeast SIR2 gene, indicate that it plays a role in mitosis."

"Overexpression of the protein phosphatase CDC14B, but not its close homolog CDC14A, results in dephosphorylation of SIRT2 with a subsequent decrease in the abundance of SIRT2 protein."

"Sir2 forms a complex with the acetyltransferase PCAF and MyoD and, when overexpressed, retards muscle differentiation."

"Sir2 forms a complex with the acetyltransferase PCAF and MyoD"

"SirT2 and its yeast counterpart Hst2 have a strong preference for histone H4K16Ac in their deacetylation activity in vitro and in vivo."

"We have pinpointed the decrease in global levels of H4K16Ac during the mammalian cell cycle to the G2/M transition that coincides with SirT2 localization on chromatin."

"SIRT2 is phosphorylated both in vitro and in vivo on serine 368 by the cell-cycle regulator, cyclin-dependent kinase 1"

"dephosphorylated by the phosphatases CDC14A and CDC14B"

"These results indicate that SIRT2 is actively exported from the nucleus in a Crm1-dependent manner."

"During early prophase, endogenous SIRT2 became enriched at the centrosome demonstrated by its colocalization with Aurora A, a mitotic regulatory kinase also found on the centrosomes"

"Utilizying coimmunoprecipitation experiments with FLAG-tagged SIRT2 and Myc-tagged Aurora A indicate that these two proteins interact in a mutliprotein complex"

"p300 interacts with Sirt2, a member of the Sir2 family, and triggers the acetylation and subsequent down-regulation of the deacetylation activity of Sirt2"

"the acetylation of Sirt2 by p300 relieves the inhibitory effect of Sirt2 on the transcriptional activity of p53"

"FoxO1 was acetylated by dissociation from sirtuin-2 (SIRT2), a NAD(+)-dependent histone deacetylase"

"the acetylated FoxO1 bound to Atg7, an E1-like protein, to influence the autophagic process leading to cell death"

"SIRT2 interacts with p65 in the cytoplasm and deacetylates p65 in vitro and in vivo at Lys310."

"p65 is deacetylated by SIRT2 in the cytoplasm to regulate the expression of specific NF-κB-dependent genes."

"Sirt2 deacetylates Par-3, a master regulator of cell polarity"

"The deacetylation of Par-3 by Sirt2 decreases the activity of the polarity complex signaling component aPKC, thereby regulating myelin formation."

"during infection with the bacterium Listeria monocytogenes, the host deacetylase sirtuin 2 (SIRT2) translocates to the nucleus, in a manner dependent on the bacterial factor InlB"

"SIRT2 associates with the transcription start site of a subset of genes repressed during infection and deacetylates histone H3 on lysine 18 (H3K18)"

"ACLY is acetylated at lysine residues 540, 546, and 554 (3K)"

"the protein deacetylase sirtuin 2 (SIRT2) deacetylates and destabilizes ACLY"

"SIRT2 directly interacted with HIF-1α and deacetylated Lys709 of HIF-1α"

"Deacetylation of HIF-1α by SIRT2 resulted in increased binding affinity for prolyl hydroxylase 2, a key regulator of HIF-1α stability, and increased HIF-1α hydroxylation and ubiquitination."

"The catalytic efficiency (kcat/Km) for the removal of a myristoyl group is slightly higher than that for the removal of an acetyl group."

"The crystal structure of SIRT2 in complex with a thiomyristoyl peptide reveals that SIRT2 possesses a large hydrophobic pocket that can accommodate the myristoyl group."

"The thiomyristoyl group of BHJH-TM1 is accommodated by a hydrophobic pocket formed by several hydrophobic residues of SIRT2, including Ile93, Phe96, Phe119, Phe131, Leu134, Leu138, Phe143, Ile169, Phe190, Ile232, and Phe234"

"Here we present high-resolution structures of human Sirt2 in complex with highly selective drug-like inhibitors that show a unique inhibitory mechanism."

"the two-domain structure typical for sirtuins-a larger domain with a Rossmann fold and a smaller zinc-binding domain that are separated through a large groove that constitutes the active site"

"The A- and B-pocket, respectively, bind the ADPR moiety, whereas the C-pocket binds the NCA of NAD+. NAD+ is able to adopt different conformations"

"We demonstrate that the NAD+-dependent deacylase SIRT2 removes the myristoyl group"

"cells sense extracellular oxidative stimuli to decrease G6PD acetylation in a SIRT2-dependent manner. The SIRT2-mediated deacetylation and activation of G6PD stimulates PPP to supply cytosolic NADPH to counteract oxidative damage"

"The K403 acetylated G6PD is incapable of forming active dimers and displays a complete loss of activity."

"Here, we show that the loss of BubR1 levels with age is due to a decline in NAD(+) and the ability of SIRT2 to maintain lysine-668 of BubR1 in a deacetylated state, which is counteracted by the acetyltransferase CBP"

"Overexpression of SIRT2 or treatment of mice with the NAD(+) precursor nicotinamide mononucleotide (NMN) increases BubR1 abundance in vivo"

"Here we show for the first time that SIRT2 interacts with the homeobox transcription factor, HOXA10, which was identified in a two-hybrid screen. Interactions were confirmed by co-immunoprecipitation from in vitro translations as well as in human cell-free extracts."

"Taken together with mouse knockout studies, our results raise the intriguing possibility that SIRT2 plays a role in mammalian development."

"Among BEX proteins, BEX4 localizes to microtubules and spindle poles, and interacts with α-tubulin (α-TUB) and sirtuin 2 (SIRT2). The overexpression of BEX4 leads to the hyperacetylation of α-TUB by inhibiting SIRT2-mediated deacetylation."

"A subsequent pull-down assay showed that SIRT2 wild-type, but not the H187Y deacetylase activity dead mutant, formed a complex with BEX4, whereas both SIRT2 wild-type and H187Y mutants interact with α-TUB"

"Pull-down assays revealed that α-TUB bound to GST-BEX4 C and very weakly to GST-BEX4 M. Interestingly, GST-BEX4 M only formed a complex with SIRT2"

"we demonstrate that SIRT2, a NAD+-dependent protein deacetylase, removes histone Kbz both in vitro and in vivo"

"Kcat/Km for debenzoylation was approximately 1/6 of that for deacetylation"

"By examining core histone Kbz levels in Sirt2+/+ (wild-type (WT)) and Sirt2−/− (knockout (KO)) mouse embryonic fibroblast (MEF) cells, we found that loss of SIRT2 expression was associated with increased Kbz levels, especially on H3 and H2B proteins"

"the effect of Sirt2 inhibition in IBD is through regulating the gut epithelium barrier by inhibiting Arf6-mediated endocytosis of E-cadherin"