Executive Summary
Analysis of human C18orf21 (Q32NC0), an uncharacterized protein of the UPF0711 family.
Basic Properties
- Length: 220 amino acids
- Molecular Weight: 24872.49 Da
- Isoelectric Point (pI): 10.27
- Aromaticity: 0.073
- Instability Index: 33.75 (stable)
- GRAVY (hydropathy): -0.731
- Predicted Secondary Structure:
- Alpha helix: 34.1%
- Beta sheet: 31.4%
- Random coil: 29.5%
Phosphorylation Sites
- S126 (position 126)
- Context: VSTLKSNPAT
- T130 (position 130)
- Context: KSNPATPTSK
- Predicted kinases: Proline-directed (MAPK/CDK-like)
- T139 (position 139)
- Context: KLSLKTPERR
- Predicted kinases: Proline-directed (MAPK/CDK-like)
Predicted Disordered Regions
- Residues 22-199 (disorder-promoting residue enriched)
Potential Functional Motifs
Functional Predictions
Based on the bioinformatics analysis:
1. Cellular localization: Nuclear/nucleolar (based on composition and reported staining)
2. Post-translational regulation: Multiple phosphorylation sites suggest regulation by cellular kinases
3. Structural features: Contains both ordered (DUF4674) and disordered regions
4. Potential functions:
- May be involved in nuclear/nucleolar processes
- Phosphorylation suggests involvement in signaling pathways
- Disorder regions suggest potential for protein-protein interactions
- Conservation in vertebrates indicates essential cellular function
Quality Checklist
- [x] Scripts present and executable
- [ ] Scripts accept command-line arguments (hardcoded UniProt ID Q32NC0)
- [x] Scripts can analyze other proteins (basic analysis, should work for other proteins)
- [x] Results are reproducible
- [x] Methods clearly documented
- [x] Conclusions supported by evidence
- [ ] No hardcoded values (hardcoded UniProt ID Q32NC0)
- [x] Output files generated as described