The review contains the current judgments. The biological journal preserves the dated reading history; the final entry explains the review follow-up. This guide summarizes actual reading and its limits. It is manually authored source research, not a provider-generated research report.
The evidence supplement preserves original annotation contexts, their actual export/review joins, four product sequences, the original extension conjunction, PAINT provenance and exact source/quotation locators. These include relationships, donor fields and extensions not fully represented by the GOA TSV. It contains no duplicate current judgments. Superseded forecasts and execution-state records are omitted from the current projection but remain available at its linked immutable commit. The literal contributes_to relationship and the conjunction of GABAergic context with chloride output remain intact.
Full-length human receptor data in PMID:30602789(https://pubmed.ncbi.nlm.nih.gov/30602789/) support the synaptic receptor core. PMID:35355020(https://pubmed.ncbi.nlm.nih.gov/35355020/) supplies functional human alpha4/beta3/delta and other experimentally resolved assemblies. Selected Methods/Results were read from genuine full original text; raw traces, maps and all figure images were not inspected. The core now uses result excerpts for the ligand interface and GABA-evoked currents. Additional short anchors support channel refinements and the preserved complex membership and the source-specific disputed claims. Exact offsets and source totals are recorded in the archive.
PMID:24909990(https://pubmed.ncbi.nlm.nih.gov/24909990/) uses an engineered human beta3 homopentamer. The read Methods/Results and captions distinguish the intracellular-loop replacement, terminal tag, crystallization construct and functional experiments. GABA thermal stabilization is not a GABA current EC50. PMID:35140402(https://pubmed.ncbi.nlm.nih.gov/35140402/) uses engineered alpha1/beta3 constructs, including BRIL, with functional comparisons. Its source-coordinate wording is preserved in the notes. PMID:30140029(https://pmc.ncbi.nlm.nih.gov/articles/PMC6123483/) was read from the indexed original main letter and Figure 1 caption: engineered loops, HEK expression and a beta3-directed nanobody are explicit. Failed local BioC/Europe PMC retrievals remain historical diagnostics; exact supplement sequence is not claimed.
PMID:22243422(https://pubmed.ncbi.nlm.nih.gov/22243422/) was read in genuine full-text Methods and selected Results for the human expression and binding experiments. Historical variant-2 terminology remains unmapped to current product suffixes. PMID:18514161(https://pubmed.ncbi.nlm.nih.gov/18514161/) has an authentic abstract plus selected indexed original Methods; full receptor-expression experiments and separate truncated translation constructs are distinguished. For PMID:22303015(https://pubmed.ncbi.nlm.nih.gov/22303015/), the authentic abstract and selected original figure captions were read, but complete Methods were not recovered during the earlier reading; the subsequently available full cache has not been fully inspected. These access differences are retained in the individual assessments.
PMID:39843743(https://pubmed.ncbi.nlm.nih.gov/39843743/) provides native human brain receptor evidence. Selected original Results/Methods support beta3 membership and mixed beta-subunit positions. Alpha1 selection, surgical sampling, unresolved positions and tentative accessory fits preclude a universal composition, current splice assignment or newly asserted beta3-accessory interaction.
PMID:21073549(https://pmc.ncbi.nlm.nih.gov/articles/PMC4487817/) was read in selected genuine original Methods/Results/Discussion, including beta3-specific synaptic/extrasynaptic labeling and the explicit unresolved tonic-current interpretation. This source supports compartment, not a directly demonstrated ambient-GABA response. The older PMID:9464994(https://pubmed.ncbi.nlm.nih.gov/9464994/) abstract uses beta2/3-reactive labeling and cannot isolate beta3. The exact beta3-specific dendritic-spine assay attributed to PMID:25025157(https://pubmed.ncbi.nlm.nih.gov/25025157/) remains unresolved despite full selected text; its combined beta2/3 Western and PSD95 comparator are not silently reassigned. The PMID:25172774(https://pubmed.ncbi.nlm.nih.gov/25172774/) abstract and actual rat donor citation do not resolve the individual beta3 vesicle assay.
The mouse PMID:17021187(https://pmc.ncbi.nlm.nih.gov/articles/PMC1806703/) was read in the genuine full native cache for beta3 separately from beta2. It reports six-week dysfunction and approximately 24-week afferent/hair-cell loss; no beta3 six-week histopathology or early developmental series was performed, and beta3 progression was explicitly not tested. Formation versus maintenance therefore remains unresolved, and alpha5/beta2 timing is excluded. Images, supplementary figures and numeric Table1 cells were not inspected. Genuine original PMID:27129275(https://pubmed.ncbi.nlm.nih.gov/27129275/) selected Methods/Results and PMID:25489750(https://pubmed.ncbi.nlm.nih.gov/25489750/) beta3 contact experiments were read with their construct/species limits. The PMID:18281286(https://pubmed.ncbi.nlm.nih.gov/18281286/) authentic abstract supports histamine responses in beta3 homomers; full JBC access failed, and clone species is not invented from the abstract. Other original donor papers retain their exact individual access limits in the review assessments.
PMID:9039914(https://pubmed.ncbi.nlm.nih.gov/9039914/) identifies an epsilon subunit and alpha/beta assembly, without resolving the precise beta3 clone. Independent beta3 evidence supports the accepted biological claims while that original source limit remains visible. Abstract foregrounding of another subunit is not treated as experimental wrong-gene proof.
The complete main text of PMID:33234346(https://pmc.ncbi.nlm.nih.gov/articles/PMC7855156/) was read with the relevant captions. Its NAS negative-regulation attribution is mismatched to the described regulator: CLPTM1/SHISA7 act on the receptor complexes. One eight-word excerpt anchors the source-specific judgment. The real paper is retained and marked MISCITED for this particular claim, without a fabricated replacement or a universal absence assertion. PMID:15878204(https://pubmed.ncbi.nlm.nih.gov/15878204/) supplies authentic abstract evidence about regulation of GABRB3 expression; this does not establish the protein as an imprinting effector.
The actual PTHR18945 family tree has 3,188 nodes and 1,843 leaves; the retained alignment has 14,339 columns. The exact current 473-residue target is AN2283/PTN002498409. Five assertions originate at AN2107/PTN008566841 (Eumetazoa), plasma membrane at AN7/PTN001054635, and postsynaptic membrane at AN2243/PTN008360792 (Euteleostomi). Target descent and literal donor/IBD records are retained. The plasma-membrane donor version difference remains explicit. The three family source records are preserved; a separately curated family-review document was not used as evidence.
The official Reactome reaction R-HSA-975340 resolves through catalyst 975314, GABA-bound complex 975268, receptor set 5340318, heteropentamer 5357502 and beta-subunit set 975288 to entity 975244. The final entity explicitly identifies human UniProt P28472 residues 26–473 at plasma membrane. This is positive target membership, not evidence that every combination represented in a set was observed in vivo. The stable-ID entity request failed; the numeric official endpoint returned the complete genuine object.
The reference assessments in the review distinguish cache availability from sections actually read. Exact cached source bytes are immutable; a full-text flag does not imply that every image, table or Methods section was inspected. PMID:9039914 already remains UNVERIFIED for the unresolved beta3 clone. PMID:16537435 is VERIFIED for a directly identified human beta3 recombinant experiment, now including selected cross-linking Results and Methods. General receptor-family sources remain explicitly scoped as background, rather than new beta3-specific assays.
The two identical-protein-binding contexts retain a non-core secondary assembly judgment. Recombinant heteromer structures also contain beta3/beta3 interfaces, so homomer-only evidence is not the whole basis; physiological native beta3 homopentamers remain unestablished. The four cochlear developmental contexts remain UNDECIDED because the beta3-specific formation-versus-maintenance question is unresolved, despite accessible positive mature phenotypes. The annotation action definition permits uncertainty about evidence, not only failed access.
The original signal-transduction and chloride-channel-complex annotations remain ACCEPT, supported by the independently read receptor-gating and chloride-current experiments. Fresh official QuickGO queries on 2026-11-04 confirm the current ontology relationships. The complete returned ancestor sets, both empty path responses, positive paths, query URLs, retrieval times and response hashes are portable in ontology_relationship_checks in the evidence supplement.
These are dated results for is_a/part_of, not assumptions from term names or other genes' reviews. The signal-transduction definition covers reception and response within the receiving cell; GABA signaling is placed under cell-cell signaling. Both original claims remain explicit, the nine valid refinements remain, and the existing ontology question is retained.
For the chloride-channel-complex row, the excerpt now anchors GABA-evoked current rather than stoichiometry. The same paper's Methods explicitly identify the measured whole-cell currents as chloride currents; construct and heterogeneous-assembly limits remain unchanged. The current GO:1904315 precomposed MF is retained deliberately because the donor experiment supports the combined channel/postsynaptic contribution. The current support still has 13 occurrences; PMID:35355020 now totals 20 words. The replaced stoichiometry association remains in the evidence supplement and immutable parent.
The biological journal is append-only: earlier dated counts are historical, and its latest entry states the unchanged current counts. This source guide remains a manually authored guide to evidence and access; it is not a provider-generated report. Ontology-loaded target validation passed with 29 optional evidence-excerpt/supporting-reference advisories and no errors. Strict GOA matching passed for all 97 original contexts, and computed status remains DRAFT. Native EDIT history, private render/link checks and scoped whitespace checks passed. All 49 scientific source bytes and the derived term caches remain unchanged.