DDC primary-source assay and table locators

This authored note makes the evidence locators public. It is not a substitute experimental source. Publication caches remain unchanged; selected independently accessed original sections are described separately from what the caches contain.

• PMID:19703902, DOI 10.1074/jbc.M109.019950, original. In Experimental Procedures, see Cells, Pulldown Assay, In Vivo Binding Assay, Activities of Tyrosine Hydroxylase and l-Dopa Decarboxylase in Cells, and Assays for TH and DDC Activities in Vitro. The human SH-SY5Y assay supplies L-DOPA and measures dopamine by HPLC; anti-DDC antibody provides a target-directed inhibition control. In Results, Positive Regulation of TH and DDC Activities by DJ-1 through Direct Interactions describes GST-DJ-1 with radiolabeled DDC, anti-DJ-1 co-IP with an IgG control, and GST-TH versus GST binding to radiolabeled DDC. The latter refers to supplemental Figure 1B within the main Results. The separate supplementary PDF was blocked by a browser challenge; its pixels were not read. Ternary association does not by itself establish channeling or a new scaffold activity. DJ-1-mediated stimulation is not assigned as a molecular function of DDC.
• PMID:12864730, DOI 10.1042/BJ20030689, abstract. The cached abstract reports DDC–AR interaction and ligand-dependent reporter enhancement. Complete original Methods were not recovered. Curated source records IM-19503-1 and IM-19503-4 identify the human pairs; IM-19503-1 annotates AR 223–559 and DDC 328–480, whereas IM-19503-4 is cellular co-IP. IM-19503-3 is a distinct rat-AR/human-DDC fragment pull-down. Its historical rat checksum is unresolved against the current sequence. These are explicit curated-record observations, not a claim that the original full assays were read.
• PMID:19056867, DOI 10.1681/ASN.2008040406, original. See Concise Methods for pooled urine, differential centrifugation and LC-MS/MS, and the linked NHLBI urinary-exosome protein table. Locate the row with Gene Name DDC, RefSeq NP_000781: the Peptide # column is 11, and Reference is 2. These are the table's recorded values. No missing table value is recoded as a negative. The fractionation and target row support contextual detection, not extracellular catalysis or unique identification of every natural product.
• PMID:22143761, DOI 10.1073/pnas.1111456108, original. The cache is abstract-only. The independent consultation recovered and read selected Results and Materials and Methods on human apoenzyme crystallization, recombinant expression and stopped-flow PLP binding. Human apoenzyme and PLP-addition experiments are distinguished from the pig holo reference structure. Lys303/PLP chemistry supports the cofactor-dependent catalytic model, without testing every natural short splice product.

The complete abstracts of PMID:16338639, PMID:7567987, PMID:15532536 and PMID:8670114 were assessed. The first describes human AADC assays but does not expose the full substrate-specific methods; the serotonin IDA annotation is retained by curator deference. The second uses a rat insulinoma expression library while stating conserved two-substrate chemistry. The third identifies an alternative human coding transcript without establishing enzyme activity. The fourth compares pig-kidney and recombinant enzyme; it is not direct evidence of identical human product kinetics.

Short original-text anchors

These quotations are from the independently recovered originals, not from rewritten publication caches. The surrounding assay and species limits above remain applicable.

• PMID:19703902, Experimental Procedures, Activities of Tyrosine Hydroxylase and l-Dopa Decarboxylase in Cells: “DDC activities in cells were measured”. Results, Positive Regulation of TH and DDC Activities by DJ-1 through Direct Interactions: “GST-DJ-1, but not GST, bound to TH and DDC”; the same Results section describing supplemental Figure 1B states “GST-TH, but not GST, bound to DDC”. The Methods specify HPLC and L-DOPA substrate; the excerpts do not imply that the separate supplementary image was inspected.
• PMID:19056867, Concise Methods, Urinary Exosome Isolation: “Urine was collected from eight healthy humans”. In the linked NHLBI table, the exact target cells are Gene Name “DDC”, RefSeq “NP_000781”, Peptide # “11”, Reference “2”. These literal cells identify the target row; they do not resolve DDC splice products.
• PMID:22143761, Materials and Methods: “Human DDC was heterologously expressed in Escherichia coli”. Results, PLP-binding kinetics: “human DDC binds PLP with high affinity”. The original compares human apoenzyme conformations with a pig holo reference; the experiments are not measurements of every natural human splice product.

2026-10-17 verification and access clarification

The existing short original-text anchors above were checked against the separately recovered original HTML and the linked NHLBI table. The source-evidence guide, under review_followup_2026_10_17, provides the original URLs, section/paragraph locators and exact saved-source digests. The normal publication caches remain unchanged, including their abstract-only flags. These are separate source artifacts, so absence of an excerpt from a normal cache does not make the original passage unverifiable. No new quotation is added or duplicated here.