ACIN1 PN Consistency Notes
- Generated: 2026-06-18
- Project: PROTEOSTASIS
- Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
- UniProt: Q9UKV3
- AIGR review status: COMPLETE
- Review batch: proteostasis-pr-1217 (PR 1217)
- Batch change status: added
Source Files Checked
Deep Research Files
AIGR Review Snapshot
- Description: ACIN1 encodes Acinus, a nuclear RNA-processing and apoptosis-associated protein. Its best-supported core functions are as an RNA-binding component of the ASAP complex and peripheral EJC-associated splicing machinery, where it participates in RNA splicing and regulation of mRNA processing, and as a caspase-activated factor that promotes apoptotic chromatin condensation. ACIN1 is localized mainly to the nucleus, nucleoplasm, and nuclear speckles. Drosophila Acinus literature links the ortholog to basal autophagy/autophagosome maturation, but current direct human evidence supports RNA processing and apoptosis more strongly than a direct human ACIN1 autophagy function.
- Existing/core annotation action counts: ACCEPT: 22; KEEP_AS_NON_CORE: 3; MARK_AS_OVER_ANNOTATED: 12; MODIFY: 3; REMOVE: 1
PN Consistency Summary
- Consistency (deep research ↔ review YAML ↔ PN ↔ mapping): Consistent, and the review handled the tension well. The human review's core functions are RNA binding in the RNPS1–SAP18–ACIN1 ASAP complex (GO:0061574) / EJC-associated splicing (GO:0008380) and caspase-activated apoptotic chromatin condensation (GO:0030263). The PN row instead places ACIN1 in the ALP branch on the strength of Drosophila Acinus basal-autophagy work. The review's
description and a suggested_question explicitly name this divergence and conclude human evidence supports RNA processing/apoptosis over a direct autophagy role.
- Does the PN taxonomy tell a story GO misses? (NEW-annotation pressure): Yes — PN asserts a candidate conserved autophagosome-maturation role that is absent from human GOA. The review correctly does not mint a NEW autophagy term (
proposed_new_terms: []); instead it captures the PN story as a suggested_question (is the Drosophila basal-autophagy role conserved?) and two suggested_experiments (autophagic-flux assay; RNA-target mapping). This is the right altitude: PN flags a hypothesis, not a GO-annotatable human fact. No new annotation warranted now.
- Evidence alignment (did PN and the review pick the same papers?): No — deliberately divergent. PN cites two Drosophila papers (Nagy et al., acinus endocytic/autophagic trafficking; Cdk5/Ser437 eLife). The review's
supported_by cites none of these; it relies on human ASAP/EJC/apoptosis literature (PMID:12665594, 20966198, 16314458, 22388736, 27365209, 10490026). The divergence is itself the finding: PN's evidence is orthology-based and the human review intentionally does not import it as human evidence.
- Verdict: Fully consistent; exemplary handling of an orthology-driven PN inclusion. No edits needed. Template/positive-control case for the phase-1 review.
Full Consistency Review
- UniProt: Q9UKV3 · batch: proteostasis-pr-1217 · review status: COMPLETE
- PN placement:
Autophagy-Lysosome Pathway → Autophagosome closure maturation and lysosome fusion → "Specific function in autophagosome maturation and lysosome fusion unknown" (1 row, ALP branch)
- PN-node mapping: leaf group =
no_mapping ("unknown/residual" bucket); parent class = context_only / too_broad_to_propagate → GO:0016236 macroautophagy; branch = no_mapping. No GO propagates to ACIN1 from PN.
Consistency (deep research ↔ review YAML ↔ PN ↔ mapping): Consistent, and the review handled the tension well. The human review's core functions are RNA binding in the RNPS1–SAP18–ACIN1 ASAP complex (GO:0061574) / EJC-associated splicing (GO:0008380) and caspase-activated apoptotic chromatin condensation (GO:0030263). The PN row instead places ACIN1 in the ALP branch on the strength of Drosophila Acinus basal-autophagy work. The review's description and a suggested_question explicitly name this divergence and conclude human evidence supports RNA processing/apoptosis over a direct autophagy role.
Does the PN taxonomy tell a story GO misses? (NEW-annotation pressure): Yes — PN asserts a candidate conserved autophagosome-maturation role that is absent from human GOA. The review correctly does not mint a NEW autophagy term (proposed_new_terms: []); instead it captures the PN story as a suggested_question (is the Drosophila basal-autophagy role conserved?) and two suggested_experiments (autophagic-flux assay; RNA-target mapping). This is the right altitude: PN flags a hypothesis, not a GO-annotatable human fact. No new annotation warranted now.
Does PN inclusion change mapping strategy? No. ACIN1's own PN node is the residual "function unknown" group → correctly no_mapping; the class is correctly held at context_only/too_broad_to_propagate. The PN authors themselves signalled uncertainty (the group label literally says function "unknown"), so the conservative no-propagation call is well-aligned. ACIN1 is a good negative control for "membership ≠ GO assertion."
Evidence alignment (did PN and the review pick the same papers?): No — deliberately divergent. PN cites two Drosophila papers (Nagy et al., acinus endocytic/autophagic trafficking; Cdk5/Ser437 eLife). The review's supported_by cites none of these; it relies on human ASAP/EJC/apoptosis literature (PMID:12665594, 20966198, 16314458, 22388736, 27365209, 10490026). The divergence is itself the finding: PN's evidence is orthology-based and the human review intentionally does not import it as human evidence.
Verdict: Fully consistent; exemplary handling of an orthology-driven PN inclusion. No edits needed. Template/positive-control case for the phase-1 review.
PN Dossier Context
- review_batch: proteostasis-pr-1217
- review_yaml: genes/human/ACIN1/ACIN1-ai-review.yaml
- PN workbook rows: 1
PN row 1: Autophagy-Lysosome Pathway | Autophagosome closure maturation and lysosome fusion | Specific function in autophagosome maturation and lysosome fusion unknown
- UniProt: Q9UKV3
- In branches: ALP
- Notes: Drosophila ACN promotes autophagosome maturation in basal autophagy.
- PN references (titles):
- Drosophila acinus encodes a novel regulator of endocytic and autophagic trafficking | Development | The Company of Biologists
- Stress-induced Cdk5 activity enhances cytoprotective basal autophagy in Drosophila melanogaster by phosphorylating acinus at serine437 | eLife (elifesciences.org)
- PN-node mapping records (path + ancestors):
- [group] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Specific function in autophagosome maturation and lysosome fusion unknown
status=no_mapping scope= GO=[]
rationale: Reviewed as an unknown or residual PN category. The label does not provide a shared GO-mappable biological process, molecular function, or cellular component.
- [class] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion
status=context_only scope=too_broad_to_propagate GO=[GO:0016236 macroautophagy]
rationale: This class is a late macroautophagy context, but the subtree mixes docking, fusion, localization, membrane-composition, and unknown late-stage roles. The class-level relation is useful for display while propagation is restricted to narrower mechanism nodes.
- [branch] Autophagy-Lysosome Pathway
status=no_mapping scope= GO=[]
rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.
Note
This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.