Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule.
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LuxS cleaves S-ribosylhomocysteine to homocysteine and the AI-2 precursor/product in a pathway from S-adenosylmethionine.
"We show here that LuxS is the AI-2 synthase and that AI-2 is produced from S-adenosylmethionine in three enzymatic steps. The substrate for LuxS is S-ribosylhomocysteine, which is cleaved to form two products, one of which is homocysteine, and the other is AI-2."
DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli.
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An E. coli luxS mutant that could not synthesize AI-2 was used to assay AI-2-dependent transcriptional responses.
"We investigated global changes in mRNA abundance elicited by the AI-2 signaling molecule through the use of a luxS mutant that was unable to synthesize AI-2."
Interaction network containing conserved and essential protein complexes in Escherichia coli.
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The source of the DnaK IPI was a chromosome-scale affinity purification survey rather than a LuxS-directed mechanistic study.
"A total of 857 proteins, including 198 of the most highly conserved, soluble non-ribosomal proteins essential in at least one bacterial species, were tagged successfully, whereas 648 could be purified to homogeneity and their interacting protein partners identified by mass spectrometry."
Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth.
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This proteomics study assigned E. coli K-12 protein forms to cytosolic, periplasmic, inner-membrane, and outer-membrane fractions.
"2,160 were annotated and assigned to the cytosol, periplasm, inner membrane, and outer membrane by biochemical fractionation followed by two-dimensional gel electrophoresis and tandem mass spectrometry."
Protein abundance profiling of the Escherichia coli cytosol.
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Ishihama et al. identified more than a thousand proteins by mass spectrometry from E. coli cytosolic fractions.
"Using a combination of LC-MS/MS approaches with protein and peptide fractionation steps we identified 1103 proteins from the cytosolic fraction of the Escherichia coli strain MC4100."
Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production.
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The luxS genes from E. coli, S. typhimurium, and V. harveyi were identified as a homologous AI-2 production gene family.
"Here we report the identification and analysis of the gene responsible for AI-2 production in V. harveyi, S. typhimurium, and E. coli."