This is manual primary-source research. The provider requests failed, so no provider-generated research report is claimed. The annotation rationales contain the scientific judgments; this guide records what was actually inspected. Cache availability and reading scope are separate.
P84243 is the shared H3.3 product of H3-3A and H3-3B. Shared peptides, purified protein and unspecified constructs do not resolve the genomic locus. The review keeps all 147 incoming contexts, including the two exosome records that collapse in the ordinary TSV projection. Its raw context archive preserves the original machine assertions.
The S-phase extension denotes temporal overlap, not exclusive S-phase localization. The sperm and pituitary basophil-cell extensions are retained literally even where the source-specific identification remains unresolved.
| Source | Reading scope | Normal cache |
|---|---|---|
| PMID:14718166(https://doi.org/10.1016/s0092-8674(03)01064-x) | Abstract; publisher full article unavailable. | Abstract only |
| PMID:17675446(https://pmc.ncbi.nlm.nih.gov/articles/PMC1948857/) | Original Results/Figures 1, 3 and 5 and Methods inspected independently; local cache is abstract-only. | Abstract only |
| PMID:19494831(https://pmc.ncbi.nlm.nih.gov/articles/PMC2693881/) | Native cache Results/Table II and protein-expression methods, lines 105–113 and 167–175. | Full text |
| PMID:19633671(https://pmc.ncbi.nlm.nih.gov/articles/PMC3125718/) | Full Results, figures and Methods; tagged HeLa H3.3 mononucleosome purification and sequencing. | Full text |
| PMID:19834512(https://pmc.ncbi.nlm.nih.gov/articles/PMC2775176/) | Native abstract; indexed original PMC2775176 Results and Figure 4, DNMT3A ADD/HP1α competition. | Abstract only |
| PMID:20110566(https://pmc.ncbi.nlm.nih.gov/articles/PMC2840985/) | Original Results, Figure 1, Supplementary Figure S4 description and Methods; local cache is abstract-only. Interaction-specific inspection is additionally recorded in source-evidence.json. | Abstract only |
| PMID:20211137(https://pmc.ncbi.nlm.nih.gov/articles/PMC2885838/) | Native cache Results lines 113, 169–173, Figure 6 human versus mouse arms. | Full text |
| PMID:20448663(https://pmc.ncbi.nlm.nih.gov/articles/PMC2892318/) | Native abstract; indexed original PMC2892318 Results/Figure 3, lines 167–172. | Abstract only |
| PMID:20458337(https://onlinelibrary.wiley.com/doi/10.1038/icb.2010.64) | Abstract and publisher supplement listing; linked XLS target-identification tables inaccessible. | Abstract only |
| PMID:20504901(https://pmc.ncbi.nlm.nih.gov/articles/PMC2885661/) | Native abstract; indexed original PMC2885661 Results lines 134–154 and Methods 269–275. | Abstract only |
| PMID:20651253(https://pmc.ncbi.nlm.nih.gov/articles/PMC2922592) | Native abstract; indexed original PMC2922592 Results, Figure 2. | Abstract only |
| PMID:20953165(https://pmc.ncbi.nlm.nih.gov/articles/PMC3020634/) | Native abstract; indexed original PMC3020634 Figure 3 and peptide-binding/construct methods. | Abstract only |
| PMID:21274551(https://link.springer.com/article/10.1007/s00412-011-0310-4) | Abstract and publisher Supporting Data 3 caption; main article and original figure not read. | Abstract only |
| PMID:21630459(https://doi.org/10.1002/pmic.201000799) | Abstract; publisher article and linked target-identification supplement inaccessible. | Abstract only |
| PMID:21636898(https://www.rcsb.org/structure/3AV2) | Abstract, primary publisher entry and deposited human H3.3 nucleosome 3AV2; full article unavailable. | Abstract only |
| PMID:21738837(https://pubmed.ncbi.nlm.nih.gov/21738837/) | Abstract; no full-text claim. | Abstract only |
| PMID:23075851(https://pmc.ncbi.nlm.nih.gov/articles/PMC4056191/) | Native cache Results lines 64–106; human protein and cellular methods 130–180; Figures 1, 3 and 4a. | Full text |
| PMID:23142979(https://pmc.ncbi.nlm.nih.gov/articles/PMC3932182/) | Native cache Results and human expression/coimmunoprecipitation methods, lines 68–78 and 114–134. | Full text |
| PMID:24183680(https://doi.org/10.1016/j.ccr.2013.10.006) | Native abstract and actual IntAct target-pair records IM-21764-7, -8 and -9; full primary methods not recovered. | Abstract only |
| PMID:24209620(https://www.ncbi.nlm.nih.gov/research/bionlp/RESTful/pmcoa.cgi/BioC_xml/PMC3994564/ascii) | Native abstract plus full BioC original PMC3994564, selected human Results passages 33–34 and Figure 7E/F caption 130; methods passage 55. | Abstract only |
| PMID:24590075(https://pmc.ncbi.nlm.nih.gov/articles/PMC4142212/) | Native cache Results lines 120–126 and Materials 146–148, Figures 1–2. | Full text |
| PMID:24981860(https://www.sciencedirect.com/science/article/pii/S2211124714004446) | Native full abstract and publisher summary; targeted IntAct P84243/publication query gave zero records. | Abstract only |
| PMID:25416956(https://pmc.ncbi.nlm.nih.gov/articles/PMC4266588/) | Native abstract, introduction/discussion; actual IntAct IM-23318-32750, -18878 and -4514 records. | Full text |
| PMID:25615412(https://doi.org/10.1021/bi501307g) | Abstract; publisher full article unavailable. | Abstract only |
| PMID:25775162(https://pubmed.ncbi.nlm.nih.gov/25775162/) | Full Results/FRET analysis and Methods. | Full text |
| PMID:26159997(https://pubmed.ncbi.nlm.nih.gov/26159997/) | Full Results on H3.3 depletion, chromosome phenotypes and centromeric CENP-A. | Full text |
| PMID:26167883(https://pmc.ncbi.nlm.nih.gov/articles/PMC4685956/) | Native Results lines 85 and 113; human protein-expression/complex methods 181–203, Figures 1–2. | Full text |
| PMID:26439302(https://pmc.ncbi.nlm.nih.gov/articles/PMC4609290/) | Native Results lines 97–113 and experimental procedures 165, Figure 1. | Full text |
| PMID:28177753(https://pubmed.ncbi.nlm.nih.gov/28177753/) | Abstract; no full-text claim. | Abstract only |
| PMID:32296183(https://pmc.ncbi.nlm.nih.gov/articles/PMC7169983/) | Native HuRI assay rationale/method sections and actual IntAct IM-25472-49172, -61907 and -134818 records. | Full text |
| PMID:33857403(https://pmc.ncbi.nlm.nih.gov/articles/PMC8221569/) | Native Results/Figures 1–3, lines 131–183; recombinant human protein methods 333–335. | Full text |
| PMID:33961781(https://pmc.ncbi.nlm.nih.gov/articles/PMC8165030/) | Native abstract/introduction/discussion and actual IntAct target records EBI-54709640, EBI-54649790 and EBI-55006526. | Full text |
| PMID:34876591(https://pmc.ncbi.nlm.nih.gov/articles/PMC8651650/) | Full Results and Methods for constructs, co-immunoprecipitation and nuclear immunofluorescence; supplementary PDF inaccessible. | Full text |
The source evidence records the inspected PTHR11426 ancestry, dated PAINT assertions, InterPro mappings, ARBA conditions and Human Protein Atlas assay provenance. P84243 descends from each of the three incoming PTN nodes. The two kinetochore judgments concern the scope of the ancestral assertion, not a claim that the target is outside the clade. No sequence-alignment or residue-loss analysis is claimed.
All 84 Reactome records were checked against their event summaries and explicit participant paths to P84243. Nucleoplasm assignments reflect modeled histone-containing chromatin. Extracellular assignments concern released chromatin and do not establish conventional secretion. The event identities, leaf compartments and target paths are retained in the source evidence.
Six contexts remain UNDECIDED: kinetochore location and assembly; the source-specific telomere-organization assertion; sperm proteomics; and both exosome contexts. The original PMID:24981860 AP-MS assignment remains unverified, while its H3.3–DAXX claim is retained using independent evidence. They are recorded as unresolved evidence, not disproved functions. No NEW annotation is proposed.
A supported generic physical association is retained as KEEP_AS_NON_CORE under the standing ClinGen project instruction. The single MARK_AS_OVER_ANNOTATED binding decision concerns a demonstrated mutant-specific gain in its cited experiment, without denying other evidence for that pair.
Histone numbering omits initiator methionine. Deposition incorporates H3.3; its partners provide chaperone activity.