GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:23212377

Identification and characterization of a human mitochondrial NAD kinase.

Human NADK2 (C5orf33) has NAD+ kinase activity using ATP or polyphosphate and is mitochondrial; it conserves the GGDG and NE/D motifs but has Ser/Lys/Trp in place of the Ppnk NAD-site Asp/Thr/Tyr.

"In the structure of the C5orf33 protein, although the GGDG motif and NE/D short motif were completely conserved, the Asp, Thr and Tyr residues were not, corresponding instead to Ser-306, Lys-317 and Trp-319"

PMID:24847004

Mitochondrial NADP(H) deficiency due to a mutation in NADK2 causes dienoyl-CoA reductase deficiency with hyperlysinemia.

Human NADK2 loss of function lowers mitochondrial NADP(H) and impairs NADPH-dependent DECR and lysine degradation.

PMID:35868311

Crystal structure of human NADK2 reveals a dimeric organization and active site occlusion by lysine acetylation.

PMID:35733246

Structure of human NADK2 reveals atypical assembly and regulation of NAD kinases from animal mitochondria.

Reactome:R-HSA-8955030

NADK2 dimer phosphorylates NAD+ to NADP+

file:HORSE/NADK2/NADK2-bioinformatics/RESULTS.md

NADK2 horse gene-model comparison against human and mouse NADK2

A0A3Q2GYY5 is a full-length alternative gene model with a 96-aa N-terminal extension and a mouse-type 22-aa exon; catalytic motifs conserved.

file:HORSE/NADK2/NADK2-uniprot.txt

UniProt entry A0A3Q2GYY5

file:human/NADK2/NADK2-uniprot.txt

UniProt entry Q4G0N4 (human NADK2)