Gene Ontology annotation through association of InterPro records with GO terms

Current family mapping carries an apoptosis term while the literature establishes a main complex IV assembly role.

Annotation inferences using phylogenetic trees

Mitochondrial localization is experimentally grounded in the family; target-specific topology supplies the more precise replacement.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

The original mapping supplies the broad inner-membrane location.

PMID:25175347

Mutations in APOPT1, encoding a mitochondrial protein, cause cavitating leukoencephalopathy with cytochrome c oxidase deficiency.

Human variants cause complex IV deficiency; M14-derived COA8 partly rescues the patient-cell defect and oxidative stress increases the mature protein.

PMID:28514442

Architecture of the human interactome defines protein communities and disease networks.

Human COA8 bait co-purifies with CLUH in BioPlex; the assay supports association, without direct affinity or a functional mechanism.

PMID:30552096

APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS.

Human complementation and pulse-chase experiments connect COA8 to complex IV assembly and subunit stability; stress-dependent accumulation and matrix-side association are experimentally supported.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

BioPlex extends human co-complex evidence. The CLUH-specific original assertion remains separate from the COX10 association.

PMID:34800366

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

MitoCoP provides positive mitochondrial proteomic detection of COA8.

PMID:16782708

Apop-1, a novel protein inducing cyclophilin D-dependent but Bax/Bak-related channel-independent apoptosis.

Mouse apoptogenic expression, cytochrome c release, caspase activation, cyclophilin-D controls and antisense suppression support a conditional apoptosis role.

PMID:18977203

Akt activation prevents Apop-1-induced death of cells.

IGF-1 protects against Apop-1-induced death in the studied vascular smooth-muscle context via PI3K/Akt signaling.

PMID:31555154

Knockdown of APOPT1/COA8 Causes Cytochrome c Oxidase Deficiency, Neuromuscular Impairment, and Reduced Resistance to Oxidative Stress in Drosophila melanogaster.

The reviewed fly ortholog localizes to mitochondria and its depletion impairs complex IV-related phenotypes.

PMID:29577824

Cavitating Leukoencephalopathy With Posterior Predominance Caused by a Deletion in the APOPT1 Gene in an Indian Boy.

Additional human APOPT1 variants support the cavitating-leukoencephalopathy disease context.

DOI:10.1101/2024.04.02.587738

COA8 is a COX10-binding protein involved in the early biogenesis of cytochrome c oxidase

Human COA8 associates with COX10. A W174A variant retains binding and restores COX10 abundance but fails to restore heme A and complex IV, separating association from functional rescue.

file:human/COA8/COA8-source-evidence.json

COA8 source evidence and product-scope index

Compact provenance for exact interaction records, donor premises, product reconstruction, MitoCoP target and selected primary access.