Use of the ND evidence code for Gene Ontology (GO) terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Genome-wide identification of fungal GPI proteins.
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Genome-wide computational screen predicting GPI-anchored proteins across fungi; the S. pombe GPI proteome is small, and mtl3/SPBC215.13 was identified as a predicted GPI-anchored cell-surface protein, supporting its localization to the outer face of the plasma membrane.
"only 33 GPI candidates were identified"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Systematic GFP-fusion localization study; the source of the UniProt endoplasmic reticulum subcellular-location assignment for mtl3 (a secretory-pathway compartment consistent with a GPI-anchored membrane protein en route to the cell surface).
"ORFeome cloning and global analysis of protein localization"
Broad functional profiling of fission yeast proteins using phenomics and machine learning.
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Genome-wide phenomics/machine-learning profiling of S. pombe deletion mutants; the source of the curated mtl3-delta stress-response phenotypes (altered sensitivity and resistance to cell-wall/membrane and ionic stressors; reduced stationary-phase viability). mtl3 appears in the supplementary phenotype matrix rather than being discussed by name in the text.
"Broad functional profiling of fission yeast proteins using phenomics and machine learning"
Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
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Systematic RNA-stability / RNA-binding-protein study; the basis for PomBase's record of mtl3 mRNA as a regulatory target (of the zinc-finger RNA-binding protein zfs1), indicating post-transcriptional control of mtl3 expression.
"Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability"
The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway.
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Characterizes the related fission-yeast plasma-membrane cell-wall stress sensors Mtl2 and Wsc1, which signal by activating the Rho1 GTPase. Provides family/architecture context for mtl3 (a Mid2-like sensor of the same class); it does not study mtl3 itself, so its findings are contextual rather than direct evidence for mtl3.
"The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway"