Combined Automated Annotation using Multiple IEA Methods
Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens.
-
Recombinant P. aeruginosa HyPRE epimerizes 4-hydroxyproline stereoisomers but has no proline racemase activity.
"B. pseudomallei and P. aeruginosa recombinant proteins presented no measurable PRAC activity but demonstrated strong epimerization of OH-L/D-Pro behaving as genuine OH-Pro epimerases."
-
Cys88 and Cys236 are both required for epimerization.
"C88SHyPRE and C236SHyPRE single mutations induce radical loss of OH-L/D-Pro epimerization"
-
The bacterial HyPREs favour the cis-D substrate at equilibrium.
"all HyPRE enzymes showed a clear advantage to OH-D-Pro substrate."
Determination of 4-hydroxyproline-2-epimerase activity by capillary electrophoresis: A stereoselective platform for inhibitor screening of amino acid isomerases.
-
A capillary-electrophoresis platform measures 4-hydroxyproline 2-epimerase kinetics and identifies pyrrole-2-carboxylate as an inhibitor.
"pyrrole 2-carboxylic acid was found to be a selective inhibitor of 4-hydroxyproline-2-epimerase"
Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks.
-
Bacterial trans-4-hydroxy-L-proline catabolism runs through 4-hydroxyproline epimerase, cis-4-hydroxy-D-proline oxidase, a DHDPS-superfamily deaminase and an alpha-ketoglutarate semialdehyde dehydrogenase.
"can be initiated by the epimerization of t4Hyp to cis-4-hydroxy-D-proline (c4Hyp) by 4HypE, followed by reactions catalyzed by c4Hyp oxidase (a member of the DAAO superfamily), c4Hyp imino acid dehydratase/deaminase (a member of the DHDPS superfamily), and α-ketoglutarate semialdehyde dehydrogenase (a member of the aldehyde dehydrogenase superfamily)."
Molecular characterization of LhpR in control of hydroxyproline catabolism and transport in Pseudomonas aeruginosa PAO1.
-
lhpA encodes the Hyp epimerase in an L-Hyp-inducible locus regulated by LhpR, and lhp knockouts impair L-Hyp catabolism.
"The first locus includes lhpABFE encoding a Hyp epimerase (LhpA) and d-Hyp dehydrogenase (LhpBEF)"
Identification and characterization of D-hydroxyproline dehydrogenase and Delta1-pyrroline-4-hydroxy-2-carboxylate deaminase involved in novel L-hydroxyproline metabolism of bacteria: metabolic convergent evolution.
-
P. aeruginosa uses a heterododecameric D-hydroxyproline dehydrogenase, unlike the homomeric P. putida enzyme.
"the latter is a novel heterododecameric structure consisting of three different subunits"
UniProtKB entry Q9I476 (4HYPE_PSEAE)
-
Curated function, kinetics, active-site cysteines and the Rhea 21152 reaction, all tied to publications.
"further metabolized by intracellular 4-hydroxy-D-proline oxidases."