Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.
A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution.
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KT2440 pcaH/pcaG encode the two-subunit protocatechuate 3,4-dioxygenase
"deleting the genes pcaH and pcaG, which encode the two-subunit protocatechuate 3,4-dioxygenase"
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In wild-type KT2440, PCA is metabolized further through the beta-ketoadipate pathway
"native (in which PCA is metabolized via the β-ketoadipate pathway)"
Cloning, sequencing, and expression of the Pseudomonas putida protocatechuate 3,4-dioxygenase genes.
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pcaG encodes the alpha subunit and pcaH encodes the beta subunit of 3,4-PCD
"contain two successive open reading frames, designated pcaH and pcaG, corresponding to the beta and alpha subunits, respectively, of 3,4-PCD"
Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry.
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The Pseudomonas putida enzyme is an alpha4beta4 complex with one Fe3+ per alpha-beta catalytic unit
"contains 4 alpha subunits of 23,000 daltons, 4 beta subunits of 26,500 daltons, and 4 ferric irons suggesting that the enzyme is a tetramer of (alpha beta Fe+3) catalytic units"
Crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase. endogenous Fe3+ ligand displacement in response to substrate binding.
The axial tyrosinate Fe3+ ligand in protocatechuate 3,4-dioxygenase influences substrate binding and product release. evidence for new reaction cycle intermediates.
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Ferric-iron ligands in 3,4-PCD are beta-subunit residues
"is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta)"
The target for the Pseudomonas putida Crc global regulator in the benzoate degradation pathway is the BenR transcriptional regulator.
Molecular characterization of the genes pcaG and pcaH, encoding protocatechuate 3,4-dioxygenase, which are essential for vanillin catabolism in Pseudomonas sp. strain HR199.
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Closely related pcaG/pcaH homologs encode protocatechuate 3,4-dioxygenase
"revealed high degrees of homology to pcaG and pcaH, encoding the two subunits of protocatechuate 3,4-dioxygenase"
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Homologous Pseudomonas pcaGH function in an ortho-cleavage aromatic catabolic route
"vanillin is degraded through the ortho-cleavage pathway in Pseudomonas sp. strain HR199"
Deep research summary for pcaG in Pseudomonas putida KT2440
Falcon deep research report for pcaG (Q88E13) in Pseudomonas putida KT2440
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Q88E13/PP_4655 is the alpha subunit of the heteromeric protocatechuate 3,4-dioxygenase (PcaGH; EC 1.13.11.3), the canonical intradiol ring-cleaving dioxygenase of the protocatechuate branch of the beta-ketoadipate pathway.
"encodes the **α-subunit of protocatechuate 3,4-dioxygenase (PcaGH; EC 1.13.11.3)**, the canonical intradiol ring-cleaving dioxygenase of the protocatechuate (PCA) branch of the **β-ketoadipate pathway**"
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The enzyme catalyzes intradiol (ortho) cleavage of protocatechuate, producing 3-carboxy-cis,cis-muconate, matching the GO:0018578 reaction.
"catalyzes **intradiol (ortho) cleavage** of the aromatic ring of **protocatechuate (3,4-dihydroxybenzoate; PCA)**, producing **3-carboxy-cis,cis-muconate**"
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The catalytic Fe(III) of 3,4-PCD is coordinated by a 2-His/2-Tyr ligand set (a complex/holoenzyme-level cofactor property), with peroxo/anhydride intermediates captured crystallographically.
"the catalytic metal is **Fe(III)** coordinated by a **2-His/2-Tyr ligand set**, and crystallography captured **alkylperoxo and anhydride intermediates** following O2 addition"
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pcaGH is the metabolic commitment step that prevents protocatechuate accumulation; deleting it blocks ring cleavage and downstream beta-ketoadipate catabolism.
"**pcaGH is the commitment step that prevents PCA accumulation**, because once PCA is cleaved to carboxymuconate, flux is directed into the central β-ketoadipate pathway"
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ΔpcaGH knockout in KT2440 blocks PCA ring cleavage and prevents further catabolism through the native beta-ketoadipate pathway, allowing PCA accumulation.
"**ΔpcaGH** blocks PCA ring cleavage, allowing PCA accumulation and preventing further catabolism through the native β-ketoadipate pathway"
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All retrieved KT2440 evidence is consistent with PcaG acting as an intracellular (cytosolic) aromatic-catabolic enzyme; no direct microscopy localization was identified.
"All evidence in the retrieved KT2440 literature is consistent with PcaG functioning as an **intracellular (cytosolic) enzyme** in aromatic catabolism"