This guide makes the evidence behind the annotation reasons portable. The review contains 92 original contexts, one core synthesis, two natural initiation products and no NEW annotation. Full original records and actual native joins are retained in the evidence archive. Source assessments distinguish the material actually read from uninspected figures, supplements and constructs.
A reference-only supported_by link refers to the same source assessment and scope recorded here; it does not imply an additional experiment. The main activity is sequence-directed PolII regulation. Binding, occupancy, transcriptional output and downstream cellular effects are distinguished. Existing experimental attributions are preserved when an inaccessible panel limits independent verification. A title focused on another regulator is not evidence of misattribution.
10608869 original record. Original Methods/Results and Fig. 6; no image inspection. Comparable-protein EMSA controls are reported as data not shown.
18177748 original record. Human fibroblast TNC RNA/protein, reporter, GATA-site mutation, DNA-affinity and endogenous ChIP evidence agree. Transfected coding provenance is not inferred from recipient species.
19497978 original record. H295R coIP includes both endogenous forms. M147L is engineered; steroid-output knockdown targets MED1, not GATA6. ChIP and promoter tests support transcription.
19666519 original record. Human EMSA/HeLa reporters separate N466H binding from activation. E486del remains intranuclear. Mouse enhancer transgenes test sites, not GATA6 deletion; detailed SI was inaccessible.
21127043 original record. Human endothelial perturbation and mouse rescue are distinct. Growth-factor occupancy differs from hypoxia. TGFB1/2 RNA effects, mouse-promoter/rat-cell testing and conditional survival remain separate.
33082341 original record. Human SebE6E7, follicle explants and organoids supply complementary contexts. NM_005257.5 is stated without modern-product alignment; the results do not establish embryonic or uniformly promaturation effects.
9566909 original record. Human expression/nuclear observations and Xenopus reporters address different claims. The proposed transition role remains unresolved; transcript decline is not functional exclusion.
16199874 original record. Fig. 5D tests GATA6 with Hey1/2/L. Recipient HEK293T cells do not establish coding species. Reporter repression is distinct from purified affinity or natural-product exclusivity.
11889139 original record. Selected original Methods/Results distinguish human GATA6/aortic cells, murine NFATc1 and rat promoter. Deletion reagents are engineered; no coronary anatomy is inferred.
33443158 original record. Mouse lineage deletions establish SAN programming; TBX3 site-dependent reporters, ChIP and nuclear-extract EMSA support regulatory work. Exact construct and ChIP-cell details need the unavailable supplement.
25068583 original record. Methods and Fig. 3: fetal-heart cells, not an hESC model. RNA-UTR targeting and the miR10a differentiation arm remain distinct.
28473536 original record. Original S1 row 298: 143-residue eDBD, matching long residues 367–509 and short 221–363. S2 rows 8278/8283: methylated/unmethylated selection models. S3 row 692: no bisulfite data.
The assessments in the YAML identify which supporting material was actually read. The following table is an access index, not a claim of complete-paper inspection. No uninspected figure image or supplementary construct sequence is treated as verified.
| Reference | Access retained |
|---|---|
| PMID:12615657(https://pubmed.ncbi.nlm.nih.gov/12615657/) | Selected original text |
| PMID:14988427(https://pubmed.ncbi.nlm.nih.gov/14988427/) | Authentic abstract; original details limited |
| PMID:15016828(https://pubmed.ncbi.nlm.nih.gov/15016828/) | Selected original text |
| PMID:16177791(https://pubmed.ncbi.nlm.nih.gov/16177791/) | Authentic abstract; original details limited |
| PMID:16968778(https://pubmed.ncbi.nlm.nih.gov/16968778/) | Authentic abstract; original details limited |
| PMID:17626241(https://pubmed.ncbi.nlm.nih.gov/17626241/) | Authentic abstract; original details limited |
| PMID:17785913(https://pubmed.ncbi.nlm.nih.gov/17785913/) | Authentic abstract; original details limited |
| PMID:17848411(https://pubmed.ncbi.nlm.nih.gov/17848411/) | Selected original text |
| PMID:18669648(https://pubmed.ncbi.nlm.nih.gov/18669648/) | Authentic abstract; original details limited |
| PMID:18671946(https://pubmed.ncbi.nlm.nih.gov/18671946/) | Authentic abstract; original details limited |
| PMID:19413330(https://pubmed.ncbi.nlm.nih.gov/19413330/) | Authentic abstract; original details limited |
| PMID:20068231(https://pubmed.ncbi.nlm.nih.gov/20068231/) | Authentic abstract; original details limited |
| PMID:20206639(https://pubmed.ncbi.nlm.nih.gov/20206639/) | Authentic abstract; original details limited |
| PMID:20581743(https://pubmed.ncbi.nlm.nih.gov/20581743/) | Newly native selected main text; scope in reference assessment; no images or supplementary Methods |
| PMID:20631719(https://pubmed.ncbi.nlm.nih.gov/20631719/) | Authentic abstract; original details limited |
| PMID:20864106(https://pubmed.ncbi.nlm.nih.gov/20864106/) | Authentic abstract; original details limited |
| PMID:21828274(https://pubmed.ncbi.nlm.nih.gov/21828274/) | Authentic abstract; original details limited |
| PMID:22158542(https://pubmed.ncbi.nlm.nih.gov/22158542/) | Newly native selected main text; scope in reference assessment; no images or supplementary Methods |
| PMID:22750565(https://pubmed.ncbi.nlm.nih.gov/22750565/) | Authentic abstract; original details limited |
| PMID:22824924(https://pubmed.ncbi.nlm.nih.gov/22824924/) | Authentic abstract; original details limited |
| PMID:26786210(https://pubmed.ncbi.nlm.nih.gov/26786210/) | Selected original text |
| PMID:27756709(https://pubmed.ncbi.nlm.nih.gov/27756709/) | Authentic abstract; original details limited |
| PMID:28112733(https://pubmed.ncbi.nlm.nih.gov/28112733/) | Authentic abstract; original details limited |
| PMID:8975704(https://pubmed.ncbi.nlm.nih.gov/8975704/) | Authentic abstract; original details limited |
| PMID:9294001(https://pubmed.ncbi.nlm.nih.gov/9294001/) | Authentic abstract; original details limited |
| PMID:9315713(https://pubmed.ncbi.nlm.nih.gov/9315713/) | Authentic abstract; original details limited |
| PMID:9593712(https://pubmed.ncbi.nlm.nih.gov/9593712/) | Authentic abstract; original details limited |
The exact 595-residue target is PTHR10071 AN20/PTN002467704. Seven actual IBD assertions lie on its ancestral path at AN0, AN3 or AN10. The reused tree has 472 nodes, 255 leaves and a 2,602-column alignment. A family-wide NOT record is outside this target lineage. The archive preserves the literal assertions, donor fields and current incoming differences. Target self-evidence is legitimate and donor count is not a confidence score. No family review is changed.
The five incoming Reactome location rows resolve to R-HSA-452902 → human Q92908, chain 1–595, nucleoplasm. 5683888 and 5685296 identify the target in binding events; 9823952 places it in the CXCR4 regulatory complex. 9823977 is an uncertain expression event with GATA6 as output, not self-synthesis catalyst. 996755 reaches the target through GATA-protein set R-HSA-996770. These are actual official HTML participant links, not successful ContentService JSON or proof that every paralog combination was assayed.
The official 595/449 sequences are independently verified by direct FASTA and coordinate reconstruction; their interpretation is in alternative_products. Sequence identity does not assign every historical reagent to a modern product. The native review restores three literal extension atoms: acts_on_population_of CL:0002495 (fetal cardiomyocyte), part_of CL:0000071 (blood vessel endothelial cell), and part_of UBERON:0000925 (endoderm). Official RO:0012003 is the first relation; BFO:0000050 represents part_of. The original labels/relations remain separately archived.
The qualified union has 52 potential normal source paths: 39 publications, ten Reactome entries and three immutable family files. At actual base 796de36063a716a46451272f4f8d08fe7f69431f, 19 main objects were reused, one publication was copied byte-exactly from published GATA2, and 24 publications plus seven Reactome entries were acquired missing-only. The actual normal-source count is 51. R-HSA-983231 remains unavailable after its retained endpoint failure. No duplicate fetch or synthetic cache was made. A source can have externally read original text while its normal cache is abstract-only or unavailable.
Seven short supporting excerpts occur once each in the review. The ledger retains authentic source hashes and byte offsets, without repeating the excerpts here. All seven excerpts match actual native-cache bytes, with acquisition coordinates preserved separately. PMID:20581743 and PMID:22158542 text was read at the stated scope. PMID:22158542 now also provides reference-only support for conserved DNA recognition, activation and the core synthesis. No legacy GATA6 review, journal, history or quotation association was found in the qualified namespace.
Default validation passed without target advisories; strict GOA reconciliation retained all 92 originals. Computed status is COMPLETE, while the one source-specific UNDECIDED judgment remains explicit. Native history, private rendering, local links and authored whitespace checks passed. The follow-up also passed default validation without target advisories, strict reconciliation of all 92 contexts, status, native history, rendering/local links and authored whitespace checks. No integration is claimed.
The two PMID:33082341 excerpts now distinguish keratinocyte epidermal programming from sebocyte differentiation; the latter does not imply increased terminal lipid output. The seven excerpts total at most 15 words from any one source, including the two excerpts from that paper.
The core retains nucleoplasm because the exact human GATA6 participant is present in the curated SFTPA promoter-binding event R-HSA-5685296, consistent with the HPA IDA and other nuclear evidence. The HPA images were not independently inspected. Nucleus is the enclosing compartment, not a competing localization.
Both UniProt initiation products remain in alternative_products. The current functional_isoforms.type enum has no alternative-translation-initiation value; neither product is relabeled as a splice variant, cleavage product or modification state. Their existing functional descriptions retain the context-dependent comparison.
The evidence archive uses repository-relative paths for current normal sources and stable checksums plus artifact names for historical private snapshots. Those snapshots are not bundled as public files; their hashes preserve identity without claiming that a local workstation path is a portable link. Primary source URLs and experimental locators remain in this guide.