Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProt record for donkey SAT1 (A0A9L0KCT9)
UniProt Swiss-Prot record for human SAT1 (P21673)
SAT1: donkey-human sequence comparison
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Donkey and human SAT1 are 97% identical without gaps.
"The donkey sequence A0A9L0KCT9 (171 residues) aligns to human P21673 (171 residues) with 97.08% identity across 171 paired residues, without gaps."
Isolation and characterization of a cDNA clone that codes for human spermidine/spermine N1-acetyltransferase.
Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase.
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Human SAT1 uses Tyr140 as general acid and Glu92 (via water) as general base.
"suggested that Tyr140 acts as general acid and Glu92, through one or more water"
Spermidine/spermine-N1-acetyltransferase-2 (SSAT2) acetylates thialysine and is not involved in polyamine metabolism.
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The SAT2 paralog (PANTHER SF51) is not a polyamine catabolic enzyme, so polyamine N1-acetylation is a SAT1-subfamily function rather than a family-wide one.
"These results indicate that SSAT2 is not a polyamine catabolic enzyme"
SAT1 (donkey A0A9L0KCT9): paralog comparison for the 2026-10-21 re-review
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Donkey SAT1 is 97.1% identical to human SAT1 and 45.3% identical to human SAT2, with Glu92 and Tyr140 conserved.
"Human SAT1 catalytic residues (Glu92 general base, Tyr140 general acid) map to donkey Glu92 and Tyr140."