Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Pseudomonas aeruginosa O-antigen chain length is determined before ligation to lipid A core.
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wzz2 deletion leaves Wzz1-specified modality; deletion of both wzz genes gives random chain length.
"As expected, the wzz2delta strain produced LPS with modal length imparted by Wzz1, and the wzz2delta/wzz1::Gm strain produced LPS O-antigen with a non-modal (random) length."
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Wzz2 forms octamers in vivo.
"In vivo protein cross-linking followed by Western immunoblotting indicated that Wzz1 forms dimers whereas Wzz2 forms octamers."
The D3 bacteriophage α-polymerase inhibitor (Iap) peptide disrupts O-antigen biosynthesis through mimicry of the chain length regulator Wzz in Pseudomonas aeruginosa.
Conserved-residue mutations in Wzy affect O-antigen polymerization and Wzz-mediated chain-length regulation in Pseudomonas aeruginosa PAO1.
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Wzy mutations alter Wzz-mediated modality, implying Wzz-Wzy interaction.
"regulation of polymer length is affected by interaction of Wzz with Wzy"