"transfers the aminomethyl group from the H-protein intermediate to **THF**, producing **5,10-methylene-THF** and releasing **NH3**"

"is a core catalytic component of the **glycine cleavage system**"

"functions with GCS **P, H, and L** proteins; H protein carries the lipoyl-bound intermediate"

"By producing **5,10-methylene-THF**, GcvT links glycine interconversion to the **one-carbon folate pool**"

"H4F-dependent enzymes help maintain high levels of intermediates needed to feed the assimilatory serine cycle"

"the THF-dependent one-carbon network and the GCS proteins are typically **cytosolic**"

"the AM1 GcvT is expected to be **cytosolic**"

"GcvT is also a core enzyme for the **reductive glycine pathway** used in synthetic C1 assimilation"