GCAP1 is a retinal calcium sensor that regulates cGMP synthesis by photoreceptor guanylate cyclase. The cloning paper summarizes its function as “stimulates synthesis of cGMP in photoreceptors” (PMID:7983048(https://pubmed.ncbi.nlm.nih.gov/7983048/)).
The complete review retains all 39 incoming contexts, including both extension atoms. The raw archive preserves original qualifiers, dates and donor sets. The source guide and independent evidence file record the separate evidence checks. No new annotation is proposed. Native acquisition, metadata reconciliation, history and validation remain pending.
The genuine P43080 fetch and qualified missing-source acquisition are complete. Both excerpts match the actual caches. Reference metadata was reconciled from those records; independent original-reading limits remain as recorded above. Validation results will be appended after the checks finish.
Validation, strict GOA matching, history validation and rendering passed. The computed status is COMPLETE with zero review warnings. Reference validation checked 31 titles and one YAML snippet with no issues; the notes quotation was separately matched to its cache. All seven inherited sources and the ontology cache remain unchanged. The earlier pending preparation entries above are superseded by this completion record.
The human assay reference construct was E6S, myristoylated in E. coli expressing yeast N-myristoyltransferase. Its target was human RetGC1; regulation is distinct from catalysis. The calcium-dependent feedback “decelerates the cyclase in the dark” (PMID:30622141(https://pubmed.ncbi.nlm.nih.gov/30622141/)).
Human retinal sections show strong cone and slight rod outer-segment staining; bovine antibody antigen does not change the tissue species (PMID:9620085(https://pubmed.ncbi.nlm.nih.gov/9620085/)).
The curated function record states “GCAP1 inhibits RetGC activation” (UniProt P43080, CC:FUNCTION). This calcium-dependent regulation supports the broader regulator core.
The current proposal retains all incoming contexts and records the remaining compartment and inhibitor-term questions separately. Earlier preparation and completion entries remain historical records.
One localization action is refined and its declared local-record reference normalized. The optional reference flag is omitted; explicit access provenance remains in the reference assessment and source guide. All earlier journal entries remain historical provenance.
The seven supported generic binding rows are retained as non-core under the ClinGen project's standing instruction. The reviewed localization and access-provenance corrections are applied; incoming contexts and prior journal entries are preserved.
Scoped validation, strict GOA matching, reference validation, history validation and rendering passed. The computed status is DRAFT with seven generic-binding advisories, retained under the project's standing instruction. Reference validation checked 31 titles and one snippet, skipped one local-record snippet, and reported no issues. Cache lint passed. The failed package-module invocation for history validation was corrected to the installed entry point; the successful check supersedes that invocation error. These results supersede earlier status summaries for the current candidate.