PA2280 (Q9I1J5) notes — ArsH
Identity
- PA2280 is the arsH gene of the PAO1 ars cluster; UniProt places it in the ArsH family (NCBIfam
TIGR02690 resist_ArsH; InterPro IPR014063 Arsenate-R_ArsH; Pfam PF03358 FMN_red, an NADPH-dependent
FMN-containing flavodoxin-like reductase fold). PANTHER PTHR43590 "ARSENIC RESISTANCE PROTEIN ARSH".
Literature
- The PseudoCAP IDA cites PMID:22304305, which is about Synechocystis sp. PCC 6803 ArsH, as its
cached abstract states explicitly: PMID:22304305 and PMID:22304305 Nothing in the abstract or MeSH terms indicates that P. aeruginosa ArsH was assayed.
QuickGO shows UniProt annotated the Synechocystis protein (P74312) with IDA/EXP from this paper, so the
PseudoCAP row appears to be a transfer of a homolog's result recorded as IDA.
- P. aeruginosa ArsH itself was characterised by Crescente et al. 2016 (PMID:26621870): PMID:26621870 and PMID:26621870 The abstract does not name
the locus; the companion Crescente PhD thesis (Kingston University, eprints.kingston.ac.uk/33493 and
related) states that pa2280 was cloned as paArsH, that PA2280 gave higher rates with FMN, and that
NADPH was the preferred reductant; the thesis names pa2280 explicitly, so the mapping rests on it.
Abstract only cached for PMID:26621870.
- In vivo role in arsenic resistance in P. aeruginosa: not tested in the papers cached here.
Annotation decisions
- GO:0016655 (IDA PseudoCAP, PMID:22304305): MODIFY to GO:0003955 NAD(P)H dehydrogenase (quinone)
activity. The activity is right for PA2280 (PMID:26621870) but the cited paper is about the
Synechocystis protein; PSEUDOCAP WRONG_REFERENCE defect recorded.
- GO:0016655 (IBA PTN008682975): MODIFY to GO:0003955 for this gene (consistency); the node term is
appropriate for its clade.
- GO:0016491 oxidoreductase activity (IEA InterPro): MODIFY to GO:0003955.
- GO:0052873 FMN reductase (NADPH) activity (IEA ARBA): UNDECIDED. FMN is the bound cofactor of ArsH;
reduction of free FMN as a substrate has not been shown for PA2280 in the material I could read.
PseudoCAP IBA-seed assessment
QuickGO (2026-10-28): PTN008682975 passes GO:0016655 (IBA) to 9 leaves: PA2280, Gloeobacter glr0005,
Klebsormidium KFL_001110020 and KFL_001820100, and fungal ArsH-like proteins (A. fumigatus
AFUA_8G07150, AFUA_1G16120, AFUA_5G15030; A. nidulans ANIA_10571; Sclerotinia SS1G_02209). The
worklist says 10 leaves in 7 taxa.
| PTN node |
node term |
n_seeds |
leaf IBAs |
seed annotation (term, evidence, PMID) |
verdict |
rationale |
| PTN008682975 |
GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor |
1 (sole) |
10 (7 taxa); 9 in QuickGO |
GO:0016655, IDA, PMID:22304305 (PseudoCAP) |
WRONG |
The cited paper characterises Synechocystis ArsH, not PA2280 (stated in the abstract), so as recorded the seed is a homolog transfer labelled IDA. The term itself is true for PA2280 by PMID:26621870 (recombinant P. aeruginosa ArsH has NQO activity), and quinone reductase activity is shared by characterised ArsH proteins, so the IBD's content is sound for the clade; the seed should be re-cited to PMID:26621870. |
Audit 2026-10-29: confidence matched to what was read
PMID:22304305 has no PMC copy and the ACS page was not retrievable, so it
remains abstract-only. Both WRONG_REFERENCE defects stay PROBABLE: the
abstract states outright that the purified, assayed protein is Synechocystis
ArsH. Not upgraded without the full text. Reference correctness changed
from MISCITED to UNVERIFIED. No action changes.