Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Functional characterization of MigA and WapR: putative rhamnosyltransferases involved in outer core oligosaccharide biosynthesis of Pseudomonas aeruginosa.
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WapR adds the alpha-1,3-L-Rha of the O-antigen-capped core; MigA adds the alpha-1,6-L-Rha of the uncapped core.
"These results provide evidence that WapR is the alpha-1,3-rhamnosyltransferase, while MigA is the alpha-1,6-rhamnosyltransferase."
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NMR and mass spectrometry of mutant LPS show the missing rhamnose linkage in each mutant.
"In contrast, uncapped core OS from the migA mutant lacked alpha-1,6-L-Rha."
Truncation in the core oligosaccharide of lipopolysaccharide affects flagella-mediated motility in Pseudomonas aeruginosa PAO1 via modulation of cell surface attachment.
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Core-truncated LPS mutants (rmlC, migA, wapR) show reduced swimming and swarming and stronger attachment, without loss of flagella.
"LPS truncations probably alter flagella-mediated motility in P. aeruginosa by modulating cell-surface attachment but not flagella synthesis"
Differential lipopolysaccharide core capping leads to quantitative and correlated modifications of mechanical and structural properties in Pseudomonas aeruginosa biofilms.
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Core truncation in migA, wapR and rmlC mutants changes cell adhesive and cohesive forces and biofilm structure.
"truncation of core oligosaccharides enhanced both adhesive and cohesive forces by up to 10-fold"
Rhamnosyltransferase genes migA and wapR are regulated in a differential manner to modulate the quantities of core oligosaccharide glycoforms produced by Pseudomonas aeruginosa.
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wapR is negatively and migA positively regulated by RhlR/RhlI; expression tracks the capped and uncapped glycoforms.
"wapR is negatively regulated, while migA is positively regulated by RhlR/RhlI quorum sensing."
UniProt entry Q9HUG5 (Q9HUG5_PSEAE)