Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Pathway for lipid A biosynthesis in Arabidopsis thaliana resembling that of Escherichia coli.
-
atlpxa-1 null mutant has lipid X reduced to about 21% of wild type; residual lipid X suggests a second UDP-GlcNAc acylation route.
"The level of lipid X in the atlpxa-1 insertion mutant was approximately 21% of its matched wild-type control"
-
Full-length AtLpxA-GFP colocalises with MitoTracker in root cells.
"The GFP fluorescence from the full-length GFP fusions of AtLpxA and AtLpxC1.1 localized in mitochondria"
In-depth temporal transcriptome profiling reveals a crucial developmental switch with roles for RNA processing and organelle metabolism that are essential for germination in Arabidopsis.
Activity and crystal structure of Arabidopsis thaliana UDP-N-acetylglucosamine acyltransferase.
-
Purified AtLpxA catalyses R-3-hydroxymyristoyl transfer from ACP to UDP-GlcNAc in vitro.
"In vitro assays show AtLpxA transfers a 3-hydroxymyristoyl group from 3-hydroxymyristoyl-ACP to the 3-OH of UDP-GlcNAc"
-
AtLpxA complements a chromosomal E. coli lpxA deletion and supports normal lipid A synthesis.
"AtLpxA was able to functionally replace EcLpxA by supporting the biosynthesis of the expected lipid A anchor of LPS"
-
AtLpxA is a homotrimer, like EcLpxA.
"Like EcLpxA, AtLpxA is a homotrimer in aqueous solution"
Detecting Gene Ontology misannotations using taxon-specific rate ratio comparisons.
UniProt entry Q9SU91 (LPXA_ARATH)