Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Transport and pharmacological properties of nine different human Na, K-ATPase isozymes.
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Human alpha/beta combinations expressed in Xenopus oocytes form functional sodium-potassium pumps. Selected original Methods and Fig. 6 independently establish ouabain-sensitive currents for human alpha3/beta1, with uninjected-oocyte currents subtracted.
"Nine different human Na,K-ATPase
isozymes, composed of 3 alpha and beta isoforms, were expressed in Xenopus
oocytes"
All human Na(+)-K(+)-ATPase alpha-subunit isoforms have a similar affinity for cardiac glycosides.
Mutations in the Na+/K+ -ATPase alpha3 gene ATP1A3 are associated with rapid-onset dystonia parkinsonism.
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Human ATP1A3 missense variants associated with rapid-onset dystonia-parkinsonism can impair enzyme activity or stability. The accessible abstract does not resolve wild-type versus mutant ER/Golgi localization.
"impair enzyme activity or stability"
Dysfunction of ouabain-induced cardiac contractility in mice with heart-specific ablation of Na,K-ATPase beta1-subunit.
Isoform specificity of cardiac glycosides binding to human Na+,K+-ATPase alpha1beta1, alpha2beta1 and alpha3beta1.
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In human atrial cardiomyocytes, alpha3 occurs at plasma and intracellular membranes, whereas the reported T-tubule staining is restricted to alpha1 and alpha2. Separate binding measurements used human heteromers expressed in yeast.
"only
alpha(1)beta(1) and alpha(2)beta(1) are located in the T-tubuli"
Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly.
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Pathological amyloid assemblies bind alpha3 and inhibit its activity. Separately read original Results include direct human-protein SPR, human cortical staining and rat-neuron perturbations; these are kept as distinct experimental contexts.
"ASPD-binding to NAKα3
impaired NAKα3-specific activity"
Identification of a VxP Targeting Signal in the Flagellar Na+ /K+ -ATPase.
The Cellular Phase of Alzheimer's Disease.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Structural basis for gating mechanism of the human sodium-potassium pump.
ATP1A:ATP1B:FXYD exchanges 3Na+ for 2K+
ATP1A:ATP1B:FXYD binds cardiac glycosides
UniProtKB P13637: ATP1A3 (human)