The source guide records original URLs, selected Methods/Results locators and access limits. The evidence map retains every original source object, the untouched genuine seed and the actual native mapping. Validation outcomes are recorded separately below after execution.
FRRS1L has a specific role in AMPA receptor biogenesis rather than an established reductase activity. Selective native rat receptor complexes and heterologous coassembly with CPT1c support a receptor-associated ER step. The reported human construct is NM_014334 without a sequence version. Mouse GluA co-IP supplies independent receptor-association evidence. Neither assay resolves a purified binary binding interface. PMID:28675162(https://pmc.ncbi.nlm.nih.gov/articles/PMC5500892/), PMID:29276473(https://pmc.ncbi.nlm.nih.gov/articles/PMC5727121/).
Human neuronally differentiated SH-SY5Y knockdown reduces AMPA-evoked calcium influx and inward current. Rat manipulations and mouse deletion/rescue connect FRRS1L to receptor abundance and signaling, while mouse-null glycan and fractionation experiments support receptor maturation. Endo-H sensitivity measures AMPAR subunits and does not establish a glycosyltransferase activity of FRRS1L. Overexpression outcomes differ across preparations; no universal sufficiency claim is made. PMID:27236917(https://pmc.ncbi.nlm.nih.gov/articles/PMC4908178/), PMID:28675162(https://pmc.ncbi.nlm.nih.gov/articles/PMC5500892/), PMID:29276473(https://pmc.ncbi.nlm.nih.gov/articles/PMC5727121/), PMID:30692144(https://pmc.ncbi.nlm.nih.gov/articles/PMC6398485/).
The review assigns six ACCEPT, two KEEP_AS_NON_CORE, one MODIFY and four UNDECIDED decisions. Raw 0 is refined to ionotropic glutamate receptor binding. Raw 1 remains unresolved because DIC antibodies isolate intact vesicles, not a demonstrated FRRS1L–DIC pair. Raw 2–7 represent the characteristic receptor-regulatory/ER role. Raw 9–10 preserve limited surface-localization evidence. Raw 8 and 11–12 retain source-specific uncertainty about postsynaptic/synaptic localization. The 2019 abstract cannot substitute for the inaccessible localization experiment, and older rat somatic ER observations cannot refute later mouse postsynaptic ER. PMID:31604597(https://pubmed.ncbi.nlm.nih.gov/31604597/).
The older 344-residue human reference and the current 293-residue reference differ by a 51-residue N-terminal extension; the remaining sequence is identical. The official historical records resolve why published S317/Q321 coordinates exceed the current length. They do not establish an alternatively spliced natural product or identify the exact sequence of every historical clone. The historical coordinates are retained, with any current-coordinate conversion explicitly labeled. UniSave Q9P0K9, NM_014334.4.
The primary text of PMID:31604597 is needed to evaluate its exact localization assays; PMID:10603000 and PMID:27239025 were available here as verified bibliographic records without abstracts. These are access follow-ups, not assertions of source error. The biological questions concern receptor-contact interfaces, the possible transport connection to dynein, and compartment-specific functions across neuronal types. They are kept distinct from the practical task of obtaining an unread paper.
All 13 QuickGO originals, evidence codes, qualifiers, WITH/FROM groups, dates and null extensions are retained. Genuine acquisition yielded 13 exported rows and 13 native seed annotations; the actual raw-to-native mapping, including all 16 TSV columns, was verified before authoring. The typed exporter annotation_extension field contains a gene-name column and is not treated as a biological extension. All eleven normal sources were genuinely acquired; three UniProt bibliography papers were fetched separately after final accounting showed that native gene acquisition had not acquired them. The untouched seed and acquisition diagnostics were retained. The two normal PAINT IBD rows match the separately inspected target lineage and literal donors. No provider call, new quotation, annotation or alternative product is claimed. Local validation, computed status, history and rendering outcomes will be appended after execution.
Local checks passed: default validation reported zero annotation advisories, strict GOA matched all 13 contexts, and native computed status is COMPLETE. The three question rationales were preserved through a lossless schema projection after the initial validation diagnostic. The genuine CREATE history, private HTML rendering, local links and exact context/source conservation were checked. All eleven normal sources, 276 prior protected files, 51 scratch files, 17 prior branch tips and 12 existing PR links remain intact. Scientific PR review and actual merge-group CI remain future requirements.
The earlier preparation and validation paragraphs are a dated journal of the initial publication. Their planned steps are now historical; they are not claims that an unexecuted amendment passed checks. The exact mapping is public in FRRS1L-source-evidence.yaml, whose index_scheme, original_contexts, genuine_projection and reviewed_contexts define the original and native indices. In biological terms, the decisions concern GO:0044877 complex binding (IEA; GO_REF:0000107), replaced by GO:0035255 ionotropic glutamate receptor binding; GO:0045505 dynein intermediate-chain binding (IEA; GO_REF:0000107), still unresolved; GO:0005789 ER membrane, GO:0099072 receptor-level regulation and GO:1900449 glutamate-signaling regulation, retained as core; GO:0005886 plasma membrane (IEA and ISS), retained as contextual; and GO:0098794 postsynapse plus GO:0045202 synapse, still unresolved. The existing human synapse ISS is a manual sequence-similarity transfer; it must not be conflated with the separate electronic IEA row.
The negative localization result is explicit: PMID:28675162 Results, Figure 5 and electron-microscopy Methods report 815/848 particles at ER membranes in 29 sampled rat neurons and 33 at or near plasma membrane, with no staining in the sampled synaptic compartment. The distribution was mainly somatic or proximal-dendritic. The immunohistochemistry Methods specify 3–4-week-old Wistar rats, while the electron-microscopy Methods separately specify tissues from two Wistar rats without an age in that sentence. The broad located_in annotation is nonexclusive and does not assert dominant or constitutive residence. MARK_AS_OVER_ANNOTATED does not require proving universal absence; the question is whether the asserted scope exceeds its support. PMID:29276473 Figure 1F and Discussion report a minor, nonzero fraction of expressed mouse HA-FRRS1L overlapping PSD-95; most labeling was nonsynaptic. These are different preparations and expression conditions. The later mouse PMID:31604597 postsynaptic-ER assay remains uninspected. A postsynapse is part of a synapse, so the unresolved later location also bears on the broader synapse claim. The sampled negative result, distinct partial-positive expression context and uninspected later experiment do not yet settle that scope judgment, so both GO:0045202 actions remain UNDECIDED. Positive surface-expression observations support the separate non-core plasma-membrane decisions without requiring every negative localization result to overturn another context. PMID:28675162, Results “FRRS1l-containing AMPARs in brain neurons localize to the ER”, Figure 5 and Methods, PMID:29276473, Figure 1F and Discussion, PMID:31604597(https://pubmed.ncbi.nlm.nih.gov/31604597/).
The binding replacement names the supported ionotropic receptor partner; it does not assert an ontology child relationship to the original complex-binding term. UniProt Q9P0K9's SUBUNIT description of AMPAR outer-core association is explicitly by similarity to B1AXV0 (ECO:0000250), whereas its FUNCTION sentence is tied to PMID:27236917 (ECO:0000269). Those two evidence scopes are distinct. An optional in_complex addition of GO:0032281 is outside this focused amendment. The existing core already describes nascent ER receptor association; this choice does not imply that the term excludes transient assemblies. The externally inspected human SH-SY5Y knockdown results are documented through the original PMID:27236917 Figure 4 and Methods/Results locators in the source guide; the normal cache remains abstract-only. No new quotation is added under the standing campaign instruction. UniProt Q9P0K9, PMID:27236917 original.
Amendment checks passed: default validation reported zero annotation advisories, strict GOA matched all 13 contexts, and computed status remains COMPLETE. The genuine EDIT history, private rendering, local links and exact source/context conservation were checked. All eleven source objects and the prior CREATE history remain unchanged. Updated-head scientific review and actual merge-group CI remain future requirements.