UniProt entry Q96N76 (HUTU_HUMAN), Urocanate hydratase
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UniProt annotates NAD(+) as the cofactor of UROC1 and multiple conserved NAD(+)-binding residues (by similarity to bacterial urocanase P25503), consistent with the electrophilic NAD+ used in urocanase catalysis.
"Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};"
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Mutations in the urocanase gene UROC1 are associated with urocanic aciduria.
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Reports the first UROC1 mutations (p.L70P, p.R450C) in a patient with urocanic aciduria; enzyme activity assays and protein expression studies show neither variant yields a fully functional urocanase, confirming UROC1 function in L-histidine catabolism.
"Computed (in silico) predictions, protein expression studies and enzyme activity assays suggest that none of the mutations can produce a fully functional enzyme."
A reference map of the human binary protein interactome.
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Systematic human all-by-all binary interactome (HuRI) reporting ~53,000 yeast-two-hybrid interactions; the source of the single UROC1 "protein binding" (UROC1-ALX1) hit, a high-throughput result with no established biological role.
"With approximately 53,000 protein-protein interactions, HuRI has approximately four times as many such interactions"
urocanate + H2O => 4-imidazolone-5-propanoate
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Reactome reaction for the human UROC1 step: cytosolic urocanate hydratase hydrolyzes urocanate to 4-imidazolone-5-propanoate; used as the source of the TAS cytosol annotation.
"Cytosolic urocanate hydratase (UROC1) catalyzes the hydrolysis of urocanate to form 4-imidazolone-5-propanoate."