Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis.
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PEX19 binds PEX11B via yeast two-hybrid and blot overlay assays
"PEX19 binds a broad spectrum of PMPs, displays saturable PMP binding, and interacts with regions of PMPs required for their targeting to peroxisomes."
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C-terminal region of PEX11B (aa 211-259) is required for PEX19 interaction
"PEX19 binds a broad spectrum of PMPs, displays saturable PMP binding, and interacts with regions of PMPs required for their targeting to peroxisomes."
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PEX19 functions as a chaperone for newly synthesized PMPs including PEX11B
"We propose that PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane."
Analysis of mammalian peroxin interactions using a non-transcription-based bacterial two-hybrid assay.
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Bacterial two-hybrid confirms PEX11B-PEX19 interaction
"We have used a non-transcription-based bacterial two-hybrid system to analyze the interactions among a set of 12 mammalian peroxins"
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CAAX motif of PEX19 enhances affinity for PEX11B
"the CAAXmotif, and not farnesylation, of Pex19p strongly enhances its affinity for Pex11pbeta"
PEX19 is a predominantly cytosolic chaperone and import receptor for class 1 peroxisomal membrane proteins.
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PEX11B classified as a class 1 PMP imported via PEX19
"PEX19 functions as both a chaperone and an import receptor for newly synthesized PMPs."
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PEX19 acts as both chaperone and import receptor
"PEX19 binds and stabilizes newly synthesized PMPs in the cytosol, binds to multiple PMP targeting signals (mPTSs), interacts with the hydrophobic domains of PMP targeting signals, and is essential for PMP targeting and import."
Fis1, DLP1, and Pex11p coordinately regulate peroxisome morphogenesis.
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PEX11B interacts directly with FIS1 via C-terminal region
"Fis1 interacted with Pex11pbeta, by direct binding apparently involving the C-terminal region of Pex11pbeta in the interaction."
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PEX11B forms homo-oligomers; N-terminal domain is required
"the highly conserved N-terminal domain of Pex11pbeta was required for the homo-oligomerization of Pex11pbeta and indispensable for the peroxisome-proliferating activity"
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Ternary complexes of FIS1, PEX11B, and DLP1 detected by cross-linking
"ternary complexes comprising Fis1, Pex11pbeta, and DLP1 were detected by chemical cross-linking"
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N-terminal domain required for peroxisome-proliferating activity
"the highly conserved N-terminal domain of Pex11pbeta was required for the homo-oligomerization of Pex11pbeta and indispensable for the peroxisome-proliferating activity"
Targeting of hFis1 to peroxisomes is mediated by Pex19p.
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hFis1 peroxisomal targeting depends on Pex19p
"peroxisomal targeting of hFis1 depends on Pex19p, a peroxisomal membrane protein import factor"
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PEX11B is mentioned in context of peroxisomal targeting pathway
"peroxisomal targeting of hFis1 depends on Pex19p, a peroxisomal membrane protein import factor"
Defining the membrane proteome of NK cells.
The peroxisomal receptor Pex19p forms a helical mPTS recognition domain.
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Crystal structure of PEX19 C-terminal mPTS recognition domain
"The crystal structure of the folded C-terminal part of the receptor reveals a globular domain that displays a bundle of three long helices in an antiparallel arrangement."
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PEX11B used as cargo protein to demonstrate mPTS binding
"the structured alpha-helical domain binds PMP-targeting signal (mPTS) sequences with about 10 muM affinity"
PEX11 family members are membrane elongation factors that coordinate peroxisome proliferation and maintenance.
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PEX11B is a membrane elongation factor
"Ectopic expression of proteins of the PEX11 family from yeast, plant or human lead to the formation of juxtaposed elongated peroxisomes (JEPs),which is evocative of an evolutionary conserved function of these proteins in membrane tubulation."
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PEX11B forms homo- and heterodimers (with PEX11G)
"We established the homo- and heterodimerization properties of the human PEX11 proteins and their interaction with the fission factor hFis1"
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PEX11B interacts with hFis1
"We established the homo- and heterodimerization properties of the human PEX11 proteins and their interaction with the fission factor hFis1"
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Overexpression causes JEPs; excess hFis1 resolves them
"We show that excess of hFis1 but not of DRP1 is sufficient to fragment JEPs into normal round-shaped organelles"
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Peroxisome number increased from ~187 to ~405 per cell with PEX11B overexpression
"PEX11-induced JEPs represent intermediates in the process of peroxisome membrane proliferation and that hFis1 is the limiting factor for progression"
A proteome-scale map of the human interactome network.
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Maximizing binary interactome mapping with a minimal number of assays.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
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PEX11B detected as interaction partner in neurodegenerative disease interactome
"Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains."
AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor.
Expression of PEX11beta mediates peroxisome proliferation in the absence of extracellular stimuli.
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PEX11B identified as peroxisomal membrane protein
"We report here the identification and characterization of two novel human peroxisomal membrane proteins, PEX11alpha and PEX11beta."
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Overexpression sufficient to induce peroxisome proliferation
"Overexpression of the human PEX11beta gene alone was sufficient to induce peroxisome proliferation, demonstrating that proliferation can occur in the absence of extracellular stimuli and may be mediated by a single gene."
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Proliferation occurs through elongation then division
"PEX11beta induces peroxisome proliferation through a multistep process involving peroxisome elongation and segregation of PEX11beta from other peroxisomal membrane proteins, followed by peroxisome division."
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PEX11B is constitutively expressed; not responsive to peroxisome proliferators
"Levels of PEX11beta mRNA were similar in all tissues examined and were unaffected by peroxisome-proliferating agents"
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Implicated in constitutive control of peroxisome abundance
"these data implicate PEX11beta in the constitutive control of peroxisome abundance"
Peroxisome synthesis in the absence of preexisting peroxisomes.
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PEX11B used as peroxisomal membrane marker
"Similar results were observed in cells transfected with expression vectors designed to express other human PMPs, including PEX3myc (Kammerer et al., 1998), PEX10myc (Warren et al., 1998), PEX11αmyc (Schrader et al., 1998), PEX11βmyc (Schrader et al., 1998), and PEX13myc (Gould et al., 1996) (data not shown)"
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Two pathways for peroxisome formation described including PEX11-mediated division
"We propose that peroxisomes may form by either of two pathways: one that involves PEX11-mediated division of preexisting peroxisomes, and another that involves PEX16-mediated formation of peroxisomes in the absence of preexisting peroxisomes."
Deep research review of PEX11B function
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Literature synthesis covering PEX11B as the constitutively expressed PEX11 family member and primary driver of peroxisome proliferation via membrane elongation and division, its coordination with the FIS1/MFF/DRP1 fission machinery, and the PEX11B-deficiency peroxisome biogenesis disorder (OMIM 614920).
"PEX11B is the best-characterized isoform in mammals and the primary driver of constitutive peroxisome proliferation"
PEX3:PEX19:class I PMP dissociates
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PEX11B modeled as class I PMP released at peroxisomal membrane
"The PEX19:PEX3:peroxisomal membrane protein complex dissociates, yielding cytosolic PEX19, membrane-bound PEX3, and the peroxisomal membrane protein inserted in the peroxisomal membrane"
PEX19:class I PMP binds PEX3
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PEX19-PEX11B complex docks at PEX3 on peroxisomal membrane
"Cytosolic PEX19 bound to a peroxisomal membrane protein binds PEX3 which is located in the peroxisomal membrane and serves as a docking receptor for PEX19"
PEX19 binds class I peroxisomal membrane proteins
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PEX19 recognizes PEX11B as class I PMP in cytosol
"In the cytosol, PEX19 binds newly synthesized class I peroxisomal membrane proteins"