Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Bus, a bushy Arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates.
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Identifies CYP79F2 as the tandem neighbour of CYP79F1 and shows that the N-terminal 62 residues of each target GFP to the ER; CYP79F2 cannot compensate for loss of CYP79F1 in short-chain glucosinolate synthesis.
"Comparison of the fluorescence patterns between the controls and the fusion proteins revealed that the GFP fusions of CYP79F1 and CYP79F2 were both located in the ER"
CYP79F1 and CYP79F2 have distinct functions in the biosynthesis of aliphatic glucosinolates in Arabidopsis.
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Recombinant CYP79F2 (yeast) exclusively metabolizes penta- and hexahomomethionine; cyp79f2 knockouts have reduced long-chain but unaffected short-chain aliphatic glucosinolates; CYP79F2 is highly expressed in hypocotyl and roots.
"CYP79F2 is highly expressed in hypocotyl and roots, whereas CYP79F1 is strongly expressed in cotyledons, rosette leaves, stems, and siliques."
Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants.
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cyp79f2 Ds gene-trap mutants are affected mainly in the root system; the cyp79f1 cyp79f2 double knockout completely lacks aliphatic glucosinolates.
"and find these mutants to be defective mainly in the root system, consistent with a root-specific expression pattern"
Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways.
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CYP79F1 and CYP79F2 are induced by methyl jasmonate.
"CYP79F1 and CYP79F2 , which are involved in biosynthesis of aliphatic glucosinolates, were induced by MeJA."
Impact of Lepidopteran Oral Secretions on the Transcriptome of Arabidopsis thaliana.
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Caterpillar oral secretions suppress aliphatic glucosinolate gene expression, including CYP79F2.
"Expression level of MYB28 (At5g61420), CYP79F1 (At1g16410), and CYP79F2 (At1g16400) 3 h after treatment"
UniProtKB entry Q9FUY7 (CYP79F2, Hexahomomethionine N-hydroxylase)