Gene Ontology annotation through association of InterPro records with GO terms.
Annotation inferences using phylogenetic trees
Combined automated annotation using Multiple IEA Methods.
Subcellular localization predictions from SUBA
Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization.
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Subcellular fractionation of intact Arabidopsis chloroplasts confirms that CSD2 and FeSOD localize to the chloroplast.
"Additionally, subcellular fractionation studies revealed the presence of CSD2 and FeSOD protein within Arabidopsis chloroplasts."
Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts.
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CSD2 is active in the chloroplast stroma and its activity and expression increase with copper supply and depend on the envelope Cu-transporting P-type ATPase PAA1.
"CSD1 encodes a Cu/ZnSOD active in the cytosol, and CSD2 encodes a Cu/ZnSOD active in the chloroplast stroma."
High light response of the thylakoid proteome in arabidopsis wild type and the ascorbate-deficient mutant vtc2-2. A comparative proteomics study.
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Cu,Zn-SOD (spot 390) is detected in the peripheral thylakoid proteome released by mild sonication of purified thylakoids and is upregulated in the ascorbate-deficient vtc2 mutant.
"Spot 390 contained Cu,Zn SOD that was consistently more abundant in vtc2 ."
Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of miR398 and important for oxidative stress tolerance.
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miR398 directs cleavage of CSD1 and CSD2 mRNAs, and oxidative stress downregulates miR398 to promote CSD1 and CSD2 accumulation and oxidative stress tolerance.
"Here, we show that miR398 expression is downregulated transcriptionally by oxidative stresses, and this downregulation is important for posttranscriptional CSD1 and CSD2 mRNA accumulation and oxidative stress tolerance."
Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression of Arabidopsis rosette leaves.
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Mild long-term salt treatment lowers CSD2 transcript expression in Arabidopsis rosette leaves.
"In both accessions, the expression of CSD2 and CSD3 was lowered by salt."
Sucrose induction of Arabidopsis miR398 represses two Cu/Zn superoxide dismutases.
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Sucrose induces miR398 and decreases CSD1 and CSD2 mRNA and protein accumulation.
"Here we show that miR398 is positively regulated by sucrose, resulting in decreased CSD1 and CSD2 mRNA and protein accumulation."
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
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AT_CHLORO maps subplastidial protein partitioning across stroma, thylakoids, and envelope membranes in purified Arabidopsis chloroplasts.
"We went a step further into the knowledge of the chloroplast proteome by focusing, in the same set of experiments, on the localization of proteins in the stroma, the thylakoids, and envelope membranes."
A proteomic strategy for global analysis of plant protein complexes.
EGY3 mediates chloroplastic ROS homeostasis and promotes retrograde signaling in response to salt stress in Arabidopsis.
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Chloroplastic EGY3 interacts with CSD2 and stabilizes it under salt and oxidative stress to maintain chloroplastic H2O2 production and retrograde signaling.
"EGY3 interacts with chloroplastic Cu/Zn-SOD2 (CSD2) and promotes CSD2 stability under stress conditions."
UniProtKB: O78310 (SODC2_ARATH)
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CSD2 is a chloroplastic Cu/Zn superoxide dismutase with an N-terminal transit peptide (AA 1-62) that binds one copper and one zinc ion per subunit.
"SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000269|PubMed:18431481, ECO:0000269|PubMed:9765550}."
Arabidopsis thaliana GOA and TAIR miRNA-pathway and miRNA-target annotations
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TAIR GAF annotates CSD2 (AT2G28190) with GO:0035195 (IEP, PMID:16861386) despite CSD2 being the cleaved target mRNA rather than a silencing pathway component.
"tair.gaf.gz AGI_LocusCode AT2G28190 CSD2 involved_in GO:0035195 PMID:16861386 IEP P Superoxide dismutase [Cu-Zn] 2, chloroplastic protein"