Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures.
Amino acid sequence of the phosphopyridoxyl peptide from P-protein of the chicken liver glycine cleavage system.
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PLP binding site identification at Lys-35 of the phosphopyridoxyl peptide
"The pyridoxal 5'-phosphate-binding site has the His-Lys(PLP)-X structure characteristic of known pyridoxal 5'-phosphate-dependent amino acid decarboxylases, tryptophan synthase, and serine hydroxymethyltransferase."
The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria.
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GLDC purification and characterization as a homodimeric, PLP-dependent enzyme
"Glycine decarboxylase, tentatively called P-protein and considered a constituent of the glycine cleavage system, was purified to apparent homogeneity from chicken liver mitochondria. P-protein is a homodimer having a Mr = approximately 200,000 and consisting of identical subunits with Mr = approximately 100,000. Each subunit appears to contain an equimolar pyridoxal 5'-phosphate"
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Glycine binding and decarboxylation activity
"P-protein could bind glycine, showing a Kd of 33 mM for it, and could catalyze glycine decarboxylation even though the rate of decarboxylation catalyzed by P-protein alone was extremely low. The product of glycine decarboxylation was methylamine"
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Stimulation by lipoic acid
"Both glycine decarboxylation and the glycine-CO2 exchange catalyzed by P-protein were stimulated 100-fold or more by the addition of lipoic acid, which is a functional group of H-protein."
The mitochondrial glycine cleavage system. Functional association of glycine decarboxylase and aminomethyl carrier protein.
Deep research on GLDC function and glycine cleavage system
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GLDC is the P-protein of the mitochondrial glycine cleavage system
"GLDC encodes the P-protein of the mitochondrial glycine cleavage system (GCS), a PLP-dependent enzyme (EC 1.4.4.2) localized to the mitochondrial matrix"
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GCS complex composition and reaction mechanism
"GCS = P (GLDC: decarboxylase), H (GCSH: lipoyl carrier / shuttle), T (AMT: aminomethyltransferase), L (DLD: dihydrolipoyl dehydrogenase). Glycine (substrate) is decarboxylated to CO2; the aminomethyl moiety is transferred to the lipoyl-H-protein"