TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle.
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Msed_0639 encodes MCE, adjacent to the MCM catalytic-subunit gene Msed_0638.
"the gene encoding MCE (Msed_0639) is adjacent to that encoding the catalytic subunit of MCM-α (Msed_0638)"
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The genes are transcriptionally induced under autotrophic growth.
"The expression of all three genes was significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO(2) fixation."
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Recombinant MCE is activated by divalent cations.
"MCE was activated by divalent cations"
Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota.
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Places the epimerase and mutase steps in the hydroxypropionate/hydroxybutyrate cycle but not in the dicarboxylate/hydroxybutyrate cycle.
"the conversion of methylmalonyl-CoA to succinyl-CoA, which is catalyzed by methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase (MCM)"
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
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Defines the 3HP/4HB cycle in M. sedula.
"In this system, one acetyl-CoA and two bicarbonate molecules were reductively converted via 3-hydroxypropionate to succinyl-CoA."
Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO(2) fixation cycle in extremely thermoacidophilic archaea.
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The cycle kinetic model uses the MCE characterization of Han et al. 2012.
"characterization of MCE and MCM was reported previously (Han et al., 2012)"
UniProt record A4YEG2 (A4YEG2_METS5)