Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Identification of a primary target of thalidomide teratogenicity.
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CRBN forms a CRL4 E3 ubiquitin ligase with DDB1 and Cul4A required for limb outgrowth and FGF8 expression; thalidomide binds CRBN and inhibits this ligase activity.
"CRBN forms an E3 ubiquitin ligase complex with damaged DNA binding protein 1 (DDB1) and Cul4A that is important for limb outgrowth and expression of the fibroblast growth factor Fgf8 in zebrafish and chicks."
Structure of the human Cereblon-DDB1-lenalidomide complex reveals basis for responsiveness to thalidomide analogs.
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The Cul4-Rbx1-DDB1-Cereblon E3 ubiquitin ligase is the target of IMiDs, which bind CRBN and promote recruitment of IKZF1/IKZF3 for ubiquitination and degradation.
"These drugs directly bind Cereblon (CRBN) and promote the recruitment of substrates Ikaros (IKZF1) and Aiolos (IKZF3) to the E3 complex, thus leading to substrate ubiquitination and degradation."
A proteome-scale map of the human interactome network.
Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS.
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Lenalidomide induces CRL4(CRBN)-dependent, proteasome- and cullin-dependent ubiquitination and degradation of CK1alpha.
"Lenalidomide induces the ubiquitination of casein kinase 1A1 (CK1alpha) by the E3 ubiquitin ligase CUL4-RBX1-DDB1-CRBN (known as CRL4(CRBN)), resulting in CK1alpha degradation."
Structural basis of lenalidomide-induced CK1α degradation by the CRL4(CRBN) ubiquitin ligase.
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CRBN is the substrate receptor of CRL4(CRBN); CK1alpha binding is strictly IMiD-dependent, illustrating drug-induced neosubstrate recruitment.
"We show that CK1alpha binding to CRL4(CRBN) is strictly dependent on the presence of an IMiD."
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling.
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CRBN mediates Wnt-induced, IMiD-independent degradation of CK1alpha and is required for physiological Wnt signaling.
"Wnt promotes the CRBN-dependent degradation of a subset of proteins. These substrates include Casein kinase 1alpha (CK1alpha), a negative regulator of Wnt signaling."
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Falcon deep research report for CRBN
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CRBN is a non-catalytic substrate-recognition adaptor of the CRL4(CRBN) E3 ubiquitin ligase that confers substrate specificity to the ubiquitination machinery.
"CRBN does not possess enzymatic activity itself but rather acts as an adapter protein that confers substrate specificity to the ubiquitination machinery"