Qualified human GATAD2B (HGNC:30778; Q8WXI9), including P66beta/KIAA1150 aliases, against current main and the retained bounded owner inventory. No prior gene review, gene notes or gene history exists in the checked namespace at main 21f1839c. This begins a new journal; no inherited prose or quote association is displaced.
The official record and direct FASTA define a 593-residue displayed product with no declared alternative products. All 74 original GO contexts were preserved, including raw 0’s contributes_to qualifier and three occurs_in nucleus atoms. The 74-key export is an offline forecast, not a genuine fetched seed.
Reviewed each annotation individually with primary-source and inference scope. The final private tally is 33 ACCEPT, 22 REMOVE, 11 MODIFY, seven UNDECIDED and one KEEP_AS_NON_CORE. Generic binding removals preserve positive pair evidence. The MBD3, FHL2, ZGPAT and LRF partner classes permit informative binding refinements without requiring physiological outcomes from the original interaction screen. FHL2 and ZGPAT primary partner studies were read separately; the ZGPAT historical 511-residue sequence differs from current Isoform 2 only at documented variant S61R (PMID:15692560(https://pubmed.ncbi.nlm.nih.gov/15692560/), PMID:19644445(https://pubmed.ncbi.nlm.nih.gov/19644445/)).
Read selected original human p66β repression and histone-association experiments and native NuRD topology. Separate p66β regulatory/structural work from CHD ATPase and HDAC catalysis, and separate beta evidence from alpha deletion experiments (PMID:16415179(https://pubmed.ncbi.nlm.nih.gov/16415179/), PMID:33283408(https://pubmed.ncbi.nlm.nih.gov/33283408/)). One core regulatory unit captures this role on chromatin. The genuine ancestral DNA-recognition inference is retained with worm fragment/assay limitations, rather than dismissed because of the human corepressor label (PMID:16159881(https://pubmed.ncbi.nlm.nih.gov/16159881/)).
Actual tree/MSA/IBD work maps the exact target to AN16/PTN002491641 beneath AN0/PTN001003231. Four inherited assertions have experimental donor grounding and no target-path loss. The self-donor entries are appropriate evidence. The donor’s sequence-selective experiment does not define a human binding motif.
Four nuclear-speckle annotations remain uncertain because the inspected early studies show nuclear puncta without establishing the GO compartment’s splicing-factor identity. Three source-specific process annotations remain uncertain despite genuine NuRD-positive experiments: Mbd3/Mbd3b and FOG1/MTA manipulations do not resolve p66β-specific participation in the inspected sections. No absence or wrong-paralog conclusion is made (PMID:11756549(https://pubmed.ncbi.nlm.nih.gov/11756549/), PMID:12183469(https://pubmed.ncbi.nlm.nih.gov/12183469/), PMID:22560079(https://pubmed.ncbi.nlm.nih.gov/22560079/), PMID:19927129(https://pubmed.ncbi.nlm.nih.gov/19927129/)).
ROOT independently reviewed all 74 scientific decisions and supplied early p66/Reactome source readings; W supplied selected original NuRD developmental/activation reading. N retained judgment ownership, read those receipts and selected passages, and distinguished original-reading scope from peer review of a receipt. Complete Methods, images or supplements are not claimed where only selected text was inspected.
The private whole contains 49 manual reference assessments and four brief active excerpts. The public evidence ledger uses hashes and offsets rather than repeating quotations. The three-source addendum adds independently read donor/partner sources to a 74-path union; no normal acquisition has occurred. GATA2’s published d82 branch remains intact while queued. Genuine native fetching, source/title/quote reconciliation, history and validation are future steps requiring the released clean-lane boundary and final-candidate review.
ROOT released reuse of the clean existing N lane before GATA2 merged, after the actual-acceptance collector was adapted to verify the preserved immutable d82 commit instead of requiring its files to remain checked out. The queued GATA2 branch, its 65 candidate objects, prior branches, scratch, shared runtime and Orca links remain protected; its real merge and executed integration are still required for acceptance.
Fresh bounded qualification at 1ca4cad1d67742cbba1467027237ef615744a0d8 found no namespace or competing source delta. All 74 source paths are now present: 53 main objects and the exact W-published R-HSA-9976102 object are unchanged, and eight publications plus twelve Reactome entries were acquired missing-only. PMID:11756549 is now a full native cache, already covered by selected original reading; PMID:12183469 remains abstract-only. No new scientific inference follows from an availability flag.
The genuine target fetch produced 74 untouched seed rows. Every original context, all 16 exported columns, the literal contributes_to relationship and the 593-residue displayed sequence match the approved forecast. Three original nucleus atoms are restored as flat BFO:0000066 extensions. No alternative product, synthetic row or NEW annotation is introduced. The 49 assessments, one core and all 74 scientific judgments are preserved; the PMID:11756549 title is reconciled to actual native spelling. All four brief excerpts now have genuine portable cache locators, preserving earlier acquisition coordinates separately.
Genuine history, targeted checks, private rendering and final status follow. Publication and actual integration are not claimed.
Target validation passed with one advisory that core GO:0003714 is not represented as an annotation row; strict GOA matching passed for all 74 original contexts. Computed status is DRAFT. The reviewed core is retained without adding a NEW annotation solely to suppress that advisory. All seven UNDECIDED judgments retain their individual evidence limits. The expected native INITIALIZED-to-DRAFT report-only exit 1 is preserved and resolved by recording the computed status. The dependency deprecation warning is separate. All 74 scientific sources and the term cache remain unchanged. Genuine CREATE history and private render/link/whitespace checks accompany this candidate. Publication and actual integration are not claimed.
Added a directly supported transcription corepressor MF assertion and explicit assessments for four already cached bibliography sources. Human GAND and fly neuronal phenotypes are distinguished in the biological summary. The source guide provides the compact current account.
Reinspection of PMID:11756549 and PMID:16415179 supports corepressor recruitment while leaving the human DNA-recognition mechanism and precise punctate compartment unresolved. The inherited DNA-function judgments and four nuclear-speck uncertainties remain unchanged. The earlier workflow entries and all original contexts remain preserved; no source bytes were rewritten. Affected amendment checks are pending.
Target validation passed without annotation advisories, and strict GOA matching passed for all 74 original contexts plus the declared NEW row. Computed review status is COMPLETE; seven source-specific UNDECIDED judgments remain. The prior DRAFT-to-COMPLETE report-only diagnostic is retained. The independently supported NEW molecular-function row resolves the former core-coverage advisory; it does not change the original 74 actions. Genuine EDIT history, private rendering/link checks and scoped authored whitespace accompany this amendment. All 74 source objects and the term cache are unchanged. Publication and renewed remote review/build are separate from these local results.
Re-read the full PMID:11756549 Discussion and separated its canonical-GATA comparison from the non-GATA p66-family inference. The source guide centralizes this distinction and the human construct linkage. Positive DNA-row support now uses the actual ancestral evidence, and the expert question asks whether direct human recognition occurs. All actions remain unchanged. COMPLETE records completion of manual adjudication, including seven explicit UNDECIDED judgments; it does not mean that every biological uncertainty is resolved. Earlier journal entries remain historical.
The direct human histone-tail interaction is now represented as a distinct MF within NuRD corepression (PMID:16415179(https://pubmed.ncbi.nlm.nih.gov/16415179/)). The source guide and annotation retain the assay and paralog limits.