Gene Ontology annotation through association of InterPro records with GO terms
A proteome-scale map of the human interactome network.
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High-throughput yeast two-hybrid study identifying approximately 14,000 binary protein-protein interactions
"Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions"
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Study focused on generating reference interactome map, not individual protein characterization
"Just as reference genome sequences revolutionized human genetics, reference maps of interactome networks will be critical to fully understand genotype-phenotype relationships"
Architecture of the human interactome defines protein communities and disease networks.
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BioPlex 2.0 AP-MS study with over 56,000 candidate interactions
"BioPlex 2.0 is the largest collection of human co-complex data assembled from a single pipeline to date, containing 56,553 interactions from 10,961 proteins"
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BTB domains like those in KCTD proteins associated with Cullin-RING E3 ubiquitin ligase system
"The cullin domain was paired de novo with 15 additional domains, many of which also co-associated... Neighbors of proteins containing RBX1/2-binding cullin homology domains were enriched with BTB, BTB_2, SOCS and FBOX domains known to bind the cullin N-terminal domain"
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
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Computational framework for analyzing effects of missense mutations on protein interactions
"Here we establish an experimentally and computationally integrated approach to investigate the functional impact of missense mutations in the context of the human interactome network"
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Study focused on autism and developmental disorders
"test our approach by analyzing ~2,000 de novo missense mutations found in autism subjects and their unaffected siblings"
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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BioPlex 3.0 with 118,162 interactions in 293T cells
"BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins"
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Comparison of context-specific interactome networks
"performing AP-MS in 293T and HCT116 cells has enabled comparison of two context-specific, proteome-scale interaction networks"
Deep research summary for KCTD14
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KCTD14 BTB domain does not form stable complex with Cullin3 in AlphaFold-based modeling
"The 2024 AF survey explicitly reports "no stable complex detected" for KCTD14-Cul3 under a 5:5 symmetry assumption for the BTB-Cul3 assembly"
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KCTD14 is predicted NOT to be a canonical CRL3 adaptor based on structural analysis
"AF-based modeling found no stable Cul3 binding, arguing against a canonical CRL3 adaptor role"
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KCTD proteins typically form pentameric assemblies via BTB domains
"KCTD14 is expected to oligomerize via its BTB domain, most commonly as a homopentamer, in keeping with the family trend"
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KCTD14 clusters structurally with KCTD7 based on fold similarity
"AlphaFold-derived pseudo-phylogenetic analysis reported a specific structural pairing of KCTD7 with KCTD14"
OpenScientist evaluation of KCTD14 self-binding core-function hypothesis
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OpenScientist found that KCTD14 self-binding is supported but should not be designated as the core function.
"Self-binding is a structural prerequisite shared across the entire KCTD family that enables downstream molecular activities (Cullin3 adaptor function, Gβγ modulation, receptor scaffolding), which are the true core functions for characterized family members."
Cyberian deep research on KCTD14 function