Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A role for the yeast SWI/SNF complex in DNA replication
Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator
Generation of superhelical torsion by ATP-dependent chromatin remodeling activities
Transcription activator interactions with multiple SWI/SNF subunits
Function and selectivity of bromodomains in anchoring chromatin-modifying complexes to promoter nucleosomes
Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription
Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure
Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast
Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits
Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair
Proteome survey reveals modularity of the yeast cell machinery
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae
Swi3p controls SWI/SNF assembly and ATP-dependent H2A-H2B displacement
A SWI/SNF- and INO80-dependent nucleosome movement at the INO1 promoter
Identification of novel activation mechanisms for FLO11 regulation in Saccharomyces cerevisiae
Architecture of the SWI/SNF-nucleosome complex
Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation
Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling
Biochemical profiling of histone binding selectivity of the yeast bromodomain family
Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress
The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation
Proteomic analysis of interactors for yeast protein arginine methyltransferase Hmt1 reveals novel substrate and insights into additional biological roles
The SWI/SNF chromatin remodeling complex influences transcription by RNA polymerase I in Saccharomyces cerevisiae
Composition and Function of Mutant Swi/Snf Complexes
Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure
SWI/SNF chromatin remodelling complex contributes to clearance of cytoplasmic protein aggregates and regulates unfolded protein response in Saccharomyces cerevisiae
Cryo-EM structure of SWI/SNF complex bound to a nucleosome
Cell cycle control of the yeast HO gene cis- and trans-acting regulators
The social and structural architecture of the yeast protein interactome
Five SWI genes are required for expression of the HO gene in yeast
SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex
A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast
Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement
The yeast SNF2/SWI2 protein has DNA-stimulated ATPase activity required for transcriptional activation
TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9
Subunits of the yeast SWI/SNF complex are members of the actin-related protein (ARP) family
A new, highly conserved domain in Swi2/Snf2 is required for SWI/SNF remodeling
Swi/Snf dynamics on stress-responsive genes is governed by competitive bromodomain interactions
The AT-hook is an evolutionarily conserved auto-regulatory domain of SWI/SNF required for cell lineage priming
Energy-driven genome regulation by ATP-dependent chromatin remodellers
Swi/Snf chromatin remodeling regulates transcriptional interference and gene repression
Transcriptional activation domains interact with ATPase subunits of yeast chromatin remodelling complexes SWI/SNF, RSC and INO80
Deep research report on SWI2
Falcon deep research report on SWI2/SNF2
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SWI2/Snf2 (YOR290C; UniProt P22082) is the catalytic ATPase (motor) subunit
of the yeast SWI/SNF chromatin-remodeling complex; its core molecular function
is ATP-dependent DNA translocation on nucleosomal substrates to reposition or
destabilize nucleosomes, driving nucleosome sliding and histone ejection.
"*Saccharomyces cerevisiae* Snf2 (a.k.a. Swi2; gene YOR290C; UniProt P22082) is the catalytic ATPase (“motor”) subunit of the yeast SWI/SNF chromatin-remodeling complex. Its core molecular function is ATP-dependent DNA translocation on nucleosomal substrates to reposition or destabilize nucleosomes"
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Nucleosomal DNA is the optimal substrate that stimulates Snf2 ATP hydrolysis
above naked DNA, and the SWI/SNF-family output is nucleosome sliding and histone
ejection driven by the Snf2 motor ATPase.
"nucleosomal DNA is reported as the optimal substrate that stimulates ATP hydrolysis above naked DNA"
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The conserved SnAC (Snf2 ATP coupling) domain couples ATP hydrolysis to
remodeling; its deletion severely impairs ATPase and nucleosome-mobilizing
activity without disrupting complex integrity or nucleosome binding.
"A highly conserved SnAC (“Snf2 ATP coupling”) domain lies between the ATPase and AT-hook region and is essential for productive remodeling. In yeast, deletion or mutation of SnAC severely impairs ATPase and nucleosome-mobilizing activities, but does not abolish complex integrity, efficient nucleosome binding, or recruitment by acidic transcription activators"
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The C-terminal AT-hook module is an auto-regulatory element (not merely a DNA
tether) that strongly boosts ATPase catalytic velocity and remodeling rate;
its deletion reduces ATP hydrolysis Vmax roughly 13-14 fold.
"the C-terminal AT-hook module in yeast Snf2 is not merely a DNA tether, but an auto-regulatory element that strongly boosts ATPase catalytic velocity and remodeling rate. Deleting both AT-hooks (ΔAT) reduces ATP hydrolysis velocity ~13-fold (DNA-stimulated) and ~14-fold (nucleosome-stimulated)"
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A discrete ~70 aa N-terminal activator-binding domain (ABD, aa 238-307) of
Swi2 directly binds the transcriptional activation domains of Ino2, Gal4,
Gcn4, Rap1, Aro80 and Swi5, supporting direct activator-to-Snf2 recruitment;
the ABD is dispensable in vivo due to redundancy among SWI/SNF subunits.
"A 2024 *Current Genetics* study provides direct biochemical evidence that transcriptional activation domains can bind to the yeast SWI/SNF ATPase subunit (Swi2/Snf2) and maps a ~70 aa N-terminal “activator-binding domain” (ABD) in Swi2 spanning aa 238–307. This ABD binds both activation domains (TAD1/TAD2) of the yeast transcription factor Ino2 and also binds TADs from several unrelated activators (Gal4, Gcn4, Rap1, Aro80, Swi5) in vitro"
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Beyond activation, SWI/SNF can repress proximal promoters by remodeling
nucleosomes downstream of an active distal transcription start site, mediating
transcriptional interference - an expanded, bidirectional functional axis.
"SWI/SNF not only promotes transcription initiation through promoter remodeling, but can also remodel nucleosomes downstream of active transcription start sites (TSSs) to repress proximal promoters (TSSPROX) associated with genes that also possess a distal active TSS (TSSDIST), thereby controlling transcriptional interference"
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The C-terminal bromodomain binds acetylated N-terminal histone tails and acts
as an acetylation sensor; competitive bromodomain interactions (acetylated
histones versus acetylated Snf2 lysines) regulate Swi/Snf recruitment/release
dynamics on stress-responsive genes.
"The bromodomain is reported to bind acetylated N-terminal histone tails. Mechanistic summary notes stimulation by tetra-acetylated H3 and acetyl-H3/H4 recognition. Recent work on stress-responsive genes showed competitive bromodomain interactions regulate Swi/Snf recruitment/release dynamics"
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Snf2 function is nuclear and chromatin-associated, supported by nucleosome
engagement assays and in vivo ChIP-based chromatin occupancy profiling.
"Snf2 is functionally characterized through nucleosome binding, ATPase assays using DNA/nucleosomes, chromatin remodeling reactions, and genome-wide chromatin occupancy measurements (ChIP-based), all of which place its function in the nucleus on chromatin"